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Assay

Preferred Assay Title

Control Type

Auxiliary Data

Construct Library Data

Readout Genes

Investigated Gene

Sequencing Platform

698 items
  • MeasurementSet
    IGVFDS6647HVMC
    CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
    Jesse Engreitz, Stanford
    Sample Summary
    Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 11-20% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
    released
  • MeasurementSet
    IGVFDS4363PDBQ
    CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
    Jesse Engreitz, Stanford
    Sample Summary
    THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
    released
  • MeasurementSet
    IGVFDS2419JZMR
    CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus
    Jesse Engreitz, Stanford
    Sample Summary
    TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin for 76-100% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
    released
  • MeasurementSet
    IGVFDS4736IKQS
    unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
    Jesse Engreitz, Stanford
    Sample Summary
    THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
    released
  • MeasurementSet
    IGVFDS3969UCCQ
    CRISPR knockout RNA-seq integrating a guide (sgRNA) library targeting transcription start sites in GATA3
    Charles Gersbach, Duke
    Sample Summary
    CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
    released
  • MeasurementSet
    IGVFDS5041NPBP
    mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
    Jesse Engreitz, Stanford
    Sample Summary
    HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
    released
  • MeasurementSet
    IGVFDS5601DSMT
    CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
    Jesse Engreitz, Stanford
    Sample Summary
    THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
    released
  • MeasurementSet
    IGVFDS2160ZTSJ
    CRISPR knockout FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes
    Charles Gersbach, Duke
    Sample Summary
    CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
    released
  • MeasurementSet
    IGVFDS8710ZSOZ
    mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
    Jesse Engreitz, Stanford
    Sample Summary
    HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
    released
  • MeasurementSet
    IGVFDS7147ANAC
    mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
    Jesse Engreitz, Stanford
    Sample Summary
    HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
    released
  • MeasurementSet
    IGVFDS9961QTUB
    ATAC-seq
    Charles Gersbach, Duke
    Sample Summary
    GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 28 days, male, Homo sapiens treated with 4 μM GSK3 inhibition for 7 days
    released
  • MeasurementSet
    IGVFDS0885VFHW
    MPRA integrating a reporter library targeting accessible genome regions in many genomic loci
    Jay Shendure, UW
    Sample Summary
    mouse embryonic stem differentiated cell specimen induced to embryoid body for 14 days, male, Mus musculus WD44 transfected with a reporter library
    released
  • MeasurementSet
    IGVFDS3899ANMJ
    CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
    Jesse Engreitz, Stanford
    Sample Summary
    THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 90-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
    released
  • MeasurementSet
    IGVFDS5128CXIS
    untransfected prime editing Variant-EFFECTS
    Jesse Engreitz, Stanford
    Sample Summary
    THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)
    released
  • MeasurementSet
    IGVFDS9738RCWL
    CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
    Jesse Engreitz, Stanford
    Sample Summary
    Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 90-100% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
    released
  • MeasurementSet
    IGVFDS1619VKNB
    ATAC-seq integrating an expression vector library of BATF3
    Charles Gersbach, Duke
    Sample Summary
    CD8-positive, alpha-beta memory T cell, Homo sapiens transduced (lentivirus) with a expression vector library
    released
  • MeasurementSet
    IGVFDS9880SAMJ
    CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
    Jesse Engreitz, Stanford
    Sample Summary
    THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
    released
  • MeasurementSet
    IGVFDS1261RWQY
    mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
    Jesse Engreitz, Stanford
    Sample Summary
    HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
    released
  • MeasurementSet
    IGVFDS1564ZVKC
    mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
    Jesse Engreitz, Stanford
    Sample Summary
    HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
    released
  • MeasurementSet
    IGVFDS5795QOPU
    CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
    Jesse Engreitz, Stanford
    Sample Summary
    THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
    released
  • MeasurementSet
    IGVFDS9690GCYG
    CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3
    Jesse Engreitz, Stanford
    Sample Summary
    TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin D for 76-100% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
    released
  • MeasurementSet
    IGVFDS6884MESW
    CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
    Jesse Engreitz, Stanford
    Sample Summary
    Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 35-45% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
    released
  • MeasurementSet
    IGVFDS6275PORW
    CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3
    Jesse Engreitz, Stanford
    Sample Summary
    TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin C for 51-75% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
    released
  • MeasurementSet
    IGVFDS1298BFAZ
    mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
    Jesse Engreitz, Stanford
    Sample Summary
    HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
    released
  • MeasurementSet
    IGVFDS3292ERTL
    CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
    Jesse Engreitz, Stanford
    Sample Summary
    THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
    released