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Measurement Set
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IGVFDS9880SAMJ
IGVFDS9880SAMJ
released
Json view
File Set Type
experimental data
Summary
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
cas mediated mutagenesis
Sample Summaries
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of PPIF) modified with a guide library
Construct Library Sets
IGVFDS2385DHZO
Guide (sgRNA) library targeting sequence variants in a genomic locus
Protocols
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
Aliases
jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep2-FFrep2-BinB_AmpliconSequencing
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM0425HXKG
InVitroSystem
THP-1
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of PPIF) modified with a guide library
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO7359LVPI
igvf:donor_of_THP1
male
Homo sapiens
Sequencing Results (Illumina)
48 items
1
2
3
4
5
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI0500TLSN
fastq
reads
I1
1
Illumina NextSeq 550
CACATCCT:ACTG
2.1 MB
Jesse Engreitz, Stanford
validated
IGVFFI0639DLTP
fastq
reads
I2
1
Illumina NextSeq 550
CAATAGTC:ACTG
1.9 MB
Jesse Engreitz, Stanford
validated
IGVFFI0641VMHM
fastq
reads
R1
1
Illumina NextSeq 550
CTGTGGCG:ACTG
8.9 MB
Jesse Engreitz, Stanford
validated
IGVFFI1042LQUB
fastq
reads
I2
1
Illumina NextSeq 550
CGCTATGT:ACTG
1.9 MB
Jesse Engreitz, Stanford
validated
IGVFFI1549SYKV
fastq
reads
R1
1
Illumina NextSeq 550
CAGCGGTA:ACTG
9.4 MB
Jesse Engreitz, Stanford
validated
IGVFFI1575FIKC
fastq
reads
I2
1
Illumina NextSeq 550
CAGCGGTA:ACTG
2.0 MB
Jesse Engreitz, Stanford
validated
IGVFFI1837CZNX
fastq
reads
R2
1
Illumina NextSeq 550
ATAGCGTC:ACTG
12 MB
Jesse Engreitz, Stanford
validated
IGVFFI1926BWQM
fastq
reads
R1
1
Illumina NextSeq 550
ATTATGTT:ACTG
9.6 MB
Jesse Engreitz, Stanford
validated
IGVFFI1987FBOD
fastq
reads
I2
1
Illumina NextSeq 550
CGCCTTCC:ACTG
2.0 MB
Jesse Engreitz, Stanford
validated
IGVFFI2196KYUA
fastq
reads
R1
1
Illumina NextSeq 550
CAGCAAGG:ACTG
9.8 MB
Jesse Engreitz, Stanford
validated
Control File Sets
1 item
Accession
Summary
Aliases
Lab
IGVFDS5128CXIS
cas mediated mutagenesis (Variant FlowFISH)
jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-THP1-PE2_Exp4_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford
Attribution
Award
HG011972
Principal Investigator(s)
Anshul Kundaje
,
Jesse Engreitz
,
Marlene Rabinovitch
,
Lars Steinmetz
,
Michael Bassik
,
Thomas Quertermous
,
Will Greenleaf
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford