IGVFDS3292ERTL

released
File Set Type
experimental data
Summary
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus followed by DNA sequencing assay
Assay Term
Donors
Samples
Sample Summaries
  • THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with a guide library
Construct Library Sets
  • IGVFDS2385DHZO Guide (sgRNA) library targeting sequence variants in a genomic locus
Aliases
jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep1-FFrep3-BinA_AmpliconSequencing

Assay Details

Sequencing Results (Illumina)

48 items
fastq
reads
I2
1
Illumina NextSeq 550
CTACCAGG:GTCA
2.9 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I1
1
Illumina NextSeq 550
CGTTACCA:GTCA
3.2 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R2
1
Illumina NextSeq 550
ACCAACTG:GTCA
19 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I2
1
Illumina NextSeq 550
ACAGGTAT:GTCA
2.9 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R1
1
Illumina NextSeq 550
AACAATGG:GTCA
12 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R2
1
Illumina NextSeq 550
GAAGAAGT:GTCA
19 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R1
1
Illumina NextSeq 550
AGGTAAGG:GTCA
13 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R1
1
Illumina NextSeq 550
AGGTCGCA:GTCA
12 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I2
1
Illumina NextSeq 550
TCCTTGGT:GTCA
2.9 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R1
1
Illumina NextSeq 550
GAAGAAGT:GTCA
14 MB
Jesse Engreitz, Stanford
released
validated

Control File Sets

1 item
Accession
Summary
Aliases
Lab
Status
cas mediated mutagenesis (Variant FlowFISH) followed by DNA sequencing assay
  • jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-THP1-PE2_Exp4_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford
released

Attribution