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1365 items
in vitro CRISPR screen using flow cytometry
reads
fastq
CRISPR FACS screen
HepG2 cell line, male, Homo sapiens (sorting details: Sorted for Bottom 20% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
CRISPR base editing FACS screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide
in vitro CRISPR screen assay
reads
fastq
Proliferation CRISPR screen
HUDEP-2 cell line, Homo sapiens modified with CRISPR cutting SpRY, transduced (lentivirus) with a guide library
Proliferation CRISPR cutting screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide
in vitro CRISPR screen using flow cytometry
reads
fastq
CRISPR FACS screen
HepG2 cell line, male, Homo sapiens (sorting details: Sorted for Top 20% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
CRISPR base editing FACS screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide
in vitro CRISPR screen using flow cytometry
reads
fastq
Variant-EFFECTS
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
CRISPR prime editing Variant-EFFECTS sorted on the expression of PPIF integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
in vitro CRISPR screen using flow cytometry
reads
fastq
Variant-EFFECTS
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 11-20% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
CRISPR prime editing Variant-EFFECTS sorted on the expression of PPIF integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
in vitro CRISPR screen using flow cytometry
reads
fastq
CRISPR FlowFISH screen
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 60-80% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
CRISPR interference FlowFISH screen sorted on the expression of NFKBIA integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide associated with blood pressure
in vitro CRISPR screen using flow cytometry
reads
fastq
Variant-EFFECTS
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
CRISPR prime editing Variant-EFFECTS sorted on the expression of PPIF integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
in vitro CRISPR screen using flow cytometry
reads
fastq
CRISPR FlowFISH screen
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin for 76-100% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
CRISPR interference FlowFISH screen sorted on the expression of TLNRD1 integrating a guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus
in vitro CRISPR screen using flow cytometry
reads
fastq
Variant-EFFECTS
THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
bulk RNA-seq assay
reads
fastq
RNA-seq
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR knockout RNA-seq integrating a guide (sgRNA) library targeting transcription start sites in GATA3
in vitro CRISPR screen using flow cytometry
seqspec, reads
yaml, fastq
CRISPR FlowFISH screen
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
in vitro CRISPR screen using flow cytometry
reads
fastq
Variant-EFFECTS
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
CRISPR prime editing Variant-EFFECTS sorted on the expression of PPIF integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
in vitro CRISPR screen using flow cytometry
reads
fastq
CRISPR FACS screen
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)
CRISPR knockout FACS screen sorted on the expression of IL7R integrating an expression vector library of BATF3 and a guide (sgRNA) library targeting transcription start sites in many genes
single-cell RNA sequencing assay
reads
fastq
SHARE-seq
colon tissue, Mus musculus C57BL/6J
single-cell RNA sequencing assay (SHARE-seq)
single-cell RNA sequencing assay
reads
fastq
SHARE-seq
bone marrow cell (cellular sub pool: SS-PKR 1), male, Homo sapiens (37 years)
single-cell RNA sequencing assay (SHARE-seq)
single-nucleus methylcytosine and transcriptome sequencing assay
reads
fastq
snMCT-seq
fibroblast reprogrammed cell specimen induced to induced pluripotent stem cell for 3 days, Homo sapiens
snMCT-seq
in vitro CRISPR screen using flow cytometry
seqspec, reads
yaml, fastq
CRISPR FlowFISH screen
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
in vitro CRISPR screen using flow cytometry
seqspec, reads
yaml, fastq
CRISPR FlowFISH screen
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
single-nucleus RNA sequencing assay
reads, seqspec
fastq, yaml
10x multiome
left cerebral cortex tissue, male, Mus musculus C57BL/6J (10 weeks)
single-nucleus RNA sequencing assay (10x multiome)
single-nucleus RNA sequencing assay
reads, seqspec
fastq, yaml
10x multiome
left cerebral cortex tissue, male, Mus musculus C57BL/6J (10 weeks)
single-nucleus RNA sequencing assay (10x multiome)
bulk assay for transposase-accessible chromatin using sequencing
reads
fastq
ATAC-seq
GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 28 days, male, Homo sapiens treated with 4 μM GSK3 inhibition for 7 days
ATAC-seq
single-nucleus methylcytosine and transcriptome sequencing assay
reads
fastq
snMCT-seq
fibroblast reprogrammed cell specimen induced to induced pluripotent stem cell for 1 day, Homo sapiens
snMCT-seq
single-nucleus RNA sequencing assay
reads, seqspec
fastq, yaml
10x multiome
left cerebral cortex tissue, female, Mus musculus C57BL/6J (10 weeks)
single-nucleus RNA sequencing assay (10x multiome)
massively parallel reporter assay
reads
fastq
MPRA
mouse embryonic stem differentiated cell specimen induced to embryoid body for 14 days, male, Mus musculus WD44 transfected with a reporter library
MPRA integrating a reporter library targeting accessible genome regions in many genomic loci
in vitro CRISPR screen using flow cytometry
reads
fastq
Variant-EFFECTS
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 90-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
CRISPR prime editing Variant-EFFECTS sorted on the expression of PPIF integrating a guide (sgRNA) library targeting sequence variants in a genomic locus