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IGVFDS9053MHCS

released
Aliases
jesse-engreitz:analysis_set_eRZ10_teloHAEC_TLNRD1_enhancer_CRISPRi_FlowFISH_analysis
File Set Type
principal analysis
Summary
CRISPR interference FlowFISH screen targeting TLNRD1 integrating a guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus
Description
Analysis of TLNRD1 CRIPSRi enhancer screen with FlowFISH in TeloHAEC
Samples
Homo sapiens TeloHAEC cell line, modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library, sorted on expression of TLNRD1
Protocols
Attribution
Jesse Engreitz, Stanford

Samples

16 items
Sample Terms
Associated Construct Library Sets
Summary
InVitroSystem
Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 0-25% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 0-25% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 51-75% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 51-75% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 26-50% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 76-100% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 76-100% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 51-75% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 0-25% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 76-100% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_teloHAEC
female
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus

MeasurementSet Input File Sets

16 items
CRISPR interference FlowFISH screen sorted on the expression of TLNRD1 integrating a guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus
Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 76-100% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
CRISPR interference FlowFISH screen sorted on the expression of TLNRD1 integrating a guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus
Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 76-100% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
CRISPR interference FlowFISH screen sorted on the expression of TLNRD1 integrating a guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus
Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 0-25% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
CRISPR interference FlowFISH screen sorted on the expression of TLNRD1 integrating a guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus
Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 51-75% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
CRISPR interference FlowFISH screen sorted on the expression of TLNRD1 integrating a guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus
Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 0-25% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
CRISPR interference FlowFISH screen sorted on the expression of TLNRD1 integrating a guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus
Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 0-25% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
CRISPR interference FlowFISH screen sorted on the expression of TLNRD1 integrating a guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus
Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 26-50% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
CRISPR interference FlowFISH screen sorted on the expression of TLNRD1 integrating a guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus
Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 26-50% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
CRISPR interference FlowFISH screen sorted on the expression of TLNRD1 integrating a guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus
Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 0-25% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
CRISPR interference FlowFISH screen sorted on the expression of TLNRD1 integrating a guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus
Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 51-75% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library