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IGVFFI1131DJSC

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS9162RHFP(CRISPR prime editing Variant-EFFECTS targeting PPIF integrating a guide (sgRNA) library targeting sequence variants in a genomic locus with unsorted FACS input, untransfected controls)
Summary
hg19 variant effects
Analysis Step Version
/analysis-step-versions/b1581dec-2980-4a2e-8884-4e95219da4d4/
File Format
tsv
Content Type
variant effects
Aliases
jesse-engreitz:PPIF_promoter_8mer_insertion_Jurkat-NoStimulation_Variant-FlowFISH_variant_effects_file
md5sum
76b76d538eed9fc2cab3a2f18a3e8eeb
Content MD5sum
05c04c7fb2785c399a4508456eee22d7
File Size
2.8 KB
Submitted File Name
PPIF_promoter_8mer_insertion_Jurkat-NoStimulation_AllelicEffectsStats.tsv.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Jesse Engreitz, Stanford

Reference Source Details

Genome Assembly
hg19

File Format Specifications

1 item
Page
Description
Type
Download
Standardized File Format
Preview
explanation of columns in analysis summary file output by the Variant-EFFECTS pipeline
file format specification

Samples

43 items
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 11-20% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 80-90% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 0-10% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) Jurkat cell line modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 35-45% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) Jurkat cell line modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 90-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 80-90% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) Jurkat cell line modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 35-45% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library

Files This File Derives From

344 items
fastq
reads
Jesse Engreitz, Stanford
7.3 MB
validated
fastq
reads
Jesse Engreitz, Stanford
7.7 MB
validated
fastq
reads
Jesse Engreitz, Stanford
7.7 MB
validated
fastq
reads
Jesse Engreitz, Stanford
7.1 MB
validated
fastq
reads
Jesse Engreitz, Stanford
3.1 MB
validated
fastq
reads
Jesse Engreitz, Stanford
3.0 MB
validated
fastq
reads
Jesse Engreitz, Stanford
12 MB
validated
fastq
reads
Jesse Engreitz, Stanford
3.2 MB
validated
fastq
reads
Jesse Engreitz, Stanford
14 MB
validated
fastq
reads
Jesse Engreitz, Stanford
3.0 MB
validated

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
variant effects
GRCh38 variant effects
Jesse Engreitz, Stanford
2.7 KB
validated