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INDEXED
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MeasurementSet
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IGVFDS0120YHME
IGVFDS0120YHME
released
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File Set Type
experimental data
Summary
prime editing Variant FlowFISH integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
PPIF
Sample Summaries
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 0-10% expression of PPIF) modified with a guide library
Construct Library Sets
IGVFDS6377KNCH
Guide (sgRNA) library targeting sequence variants in a genomic locus
Protocols
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
Aliases
jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep2-FFrep1-BinA_AmpliconSequencing
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM4133KDLM
InVitroSystem
Jurkat
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 0-10% expression of PPIF) modified with a guide library
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO1080XFGV
igvf:donor_of_Jurkat
male
Homo sapiens
Control File Sets
1 item
Accession
Type
Summary
Aliases
Lab
IGVFDS9477ARWI
MeasurementSet
prime editing Variant FlowFISH
jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-Jurkat-PE2_Exp1_NegControl_AmpliconSequencing
jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-Jurkat-PE2_Exp1_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford
File Sets Using This Measurement Set as an Input
1 item
Accession
Type
Summary
Aliases
Lab
IGVFDS9162RHFP
AnalysisSet
principal analysis of Variant FlowFISH data
jesse-engreitz:PPIF_promoter_8mer_insertion_Jurkat-NoStimulation_Variant-FlowFISH_analysis
Jesse Engreitz, Stanford
Attribution
Award
HG011972
Principal Investigator(s)
Anshul Kundaje
,
Jesse Engreitz
,
Marlene Rabinovitch
,
Lars Steinmetz
,
Michael Bassik
,
Thomas Quertermous
,
Will Greenleaf
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford