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INDEXED
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Measurement Set
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IGVFDS0120YHME
IGVFDS0120YHME
released
Json view
File Set Type
experimental data
Summary
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
cas mediated mutagenesis
Sample Summaries
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 0-10% expression of PPIF) modified with a guide library
Construct Library Sets
IGVFDS6377KNCH
Guide (sgRNA) library targeting sequence variants in a genomic locus
Protocols
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
Aliases
jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep2-FFrep1-BinA_AmpliconSequencing
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM4133KDLM
InVitroSystem
Jurkat
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 0-10% expression of PPIF) modified with a guide library
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO1080XFGV
igvf:donor_of_Jurkat
male
Homo sapiens
Sequencing Results (Illumina)
8 items
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI0174SRME
fastq
reads
I2
1
Illumina NextSeq 550
TCTGGCGA:CAGT
3.1 MB
Jesse Engreitz, Stanford
validated
IGVFFI0523CAKB
fastq
reads
I1
1
Illumina NextSeq 550
CCTACCAT:CAGT
3.3 MB
Jesse Engreitz, Stanford
validated
IGVFFI0536RYTX
fastq
reads
I2
1
Illumina NextSeq 550
CCTACCAT:CAGT
3.2 MB
Jesse Engreitz, Stanford
validated
IGVFFI1487ILZL
fastq
reads
I1
1
Illumina NextSeq 550
TCTGGCGA:CAGT
3.1 MB
Jesse Engreitz, Stanford
validated
IGVFFI1659GJXT
fastq
reads
R2
1
Illumina NextSeq 550
TCTGGCGA:CAGT
14 MB
Jesse Engreitz, Stanford
validated
IGVFFI2987HUNV
fastq
reads
R1
1
Illumina NextSeq 550
CCTACCAT:CAGT
7.9 MB
Jesse Engreitz, Stanford
validated
IGVFFI8471SQCM
fastq
reads
R1
1
Illumina NextSeq 550
TCTGGCGA:CAGT
7.7 MB
Jesse Engreitz, Stanford
validated
IGVFFI8572LIGU
fastq
reads
R2
1
Illumina NextSeq 550
CCTACCAT:CAGT
14 MB
Jesse Engreitz, Stanford
validated
Control File Sets
1 item
Accession
Summary
Aliases
Lab
IGVFDS9477ARWI
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-Jurkat-PE2_Exp1_NegControl_AmpliconSequencing
jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-Jurkat-PE2_Exp1_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford
Attribution
Award
HG011972
Principal Investigator(s)
Anshul Kundaje
,
Jesse Engreitz
,
Marlene Rabinovitch
,
Lars Steinmetz
,
Michael Bassik
,
Thomas Quertermous
,
Will Greenleaf
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford