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IGVFDS9477ARWI

released
File Set Type
experimental data
Summary
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
cas mediated mutagenesis
Sample Summaries
  • Jurkat cell line, male, Homo sapiens modified with a guide library
Construct Library Sets
  • IGVFDS6377KNCH Guide (sgRNA) library targeting sequence variants in a genomic locus
Protocols
Aliases
jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-Jurkat-PE2_Exp1_NegControl_AmpliconSequencing
jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-Jurkat-PE2_Exp1_NegControl_AmpliconSequencing

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Jurkat cell line, male, Homo sapiens modified with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_Jurkat
male
Homo sapiens

Sequencing Results (Illumina)

8 items
fastq
reads
I2
1
Illumina NextSeq 550
GACCTAAC:GTCA
3.2 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
Illumina NextSeq 550
TTGTCTAT:ACTG
3.1 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
Illumina NextSeq 550
TTGTCTAT:ACTG
3.4 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
Illumina NextSeq 550
TTGTCTAT:ACTG
12 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
Illumina NextSeq 550
GACCTAAC:GTCA
3.4 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
Illumina NextSeq 550
GACCTAAC:GTCA
7.6 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
Illumina NextSeq 550
GACCTAAC:GTCA
12 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
Illumina NextSeq 550
TTGTCTAT:ACTG
7.5 MB
Jesse Engreitz, Stanford
validated

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford