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IGVFDS4045IKYV

released
File Set Type
experimental data
Summary
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 11-20% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
Construct Library Sets
  • IGVFDS6377KNCH guide (sgRNA) library targeting sequence variants in a genomic locus
Protocols
Aliases
jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep1-FFrep2-BinB_AmpliconSequencing

Sequencing Results (Illumina)

8 items
fastq
reads
I1
1
AGGTAAGG:CAGT
3.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
AGGTAAGG:CAGT
7.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
CTATGCGT:CAGT
3.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
AGGTAAGG:CAGT
13 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
CTATGCGT:CAGT
7.3 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
CTATGCGT:CAGT
13 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
CTATGCGT:CAGT
3.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
AGGTAAGG:CAGT
2.9 MB
Jesse Engreitz, Stanford
validated

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford