IGVFDS4045IKYV

released
File Set Type
experimental data
Summary
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
Sample Summaries
  • Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 11-20% expression of PPIF) modified with a guide library
Construct Library Sets
  • IGVFDS6377KNCH Guide (sgRNA) library targeting sequence variants in a genomic locus
Aliases
jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep1-FFrep2-BinB_AmpliconSequencing

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 11-20% expression of PPIF) modified with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_Jurkat
male
Homo sapiens

Assay Details

Sequencing Results (Illumina)

8 items
fastq
reads
I1
1
Illumina NextSeq 550
AGGTAAGG:CAGT
3.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
Illumina NextSeq 550
AGGTAAGG:CAGT
7.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
Illumina NextSeq 550
CTATGCGT:CAGT
3.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
Illumina NextSeq 550
AGGTAAGG:CAGT
13 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
Illumina NextSeq 550
CTATGCGT:CAGT
7.3 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
Illumina NextSeq 550
CTATGCGT:CAGT
13 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
Illumina NextSeq 550
CTATGCGT:CAGT
3.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
Illumina NextSeq 550
AGGTAAGG:CAGT
2.9 MB
Jesse Engreitz, Stanford
validated

Control File Sets

1 item
Accession
Summary
Aliases
Lab
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-Jurkat-PE2_Exp1_NegControl_AmpliconSequencing
  • jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-Jurkat-PE2_Exp1_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford

Attribution