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Assay
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in vitro CRISPR screen using flow cytometry
41
Preferred Assay Title
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None
Variant-EFFECTS
41
Control Type
All
None
unsorted FACS input
8
untransfected
1
Construct Library Data
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None
guide library
40
Readout Genes
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None
PPIF
32
Sequencing Platform
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None
Illumina NextSeq 550
41
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/analysis-sets/IGVFDS5031MNRR/
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Taxa
UUID
/measurement-sets/IGVFDS4736IKQS/
IGVFDS4736IKQS
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep4-FACS_Input_AmpliconSequencing
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM0906STHP/
released
unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
fe6d143f-dbd2-40a5-b72b-55021f81951f
/measurement-sets/IGVFDS5601DSMT/
IGVFDS5601DSMT
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep2-BinB_AmpliconSequencing
PPIF
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM1683OFWB/
released
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
fd6e5d84-173b-4f03-8de0-67bbec8d433f
/measurement-sets/IGVFDS5405RYGU/
IGVFDS5405RYGU
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep1-BinD_AmpliconSequencing
PPIF
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM6331RKYX/
released
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
f016c775-b215-4660-b119-5a7f3b736144
/measurement-sets/IGVFDS8504HJPW/
IGVFDS8504HJPW
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep1-BinA_AmpliconSequencing
PPIF
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM0841TQDY/
released
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
ef5a9a11-b681-40fb-acb0-401964b44f23
/measurement-sets/IGVFDS6071XLAA/
IGVFDS6071XLAA
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep3-BinA_AmpliconSequencing
PPIF
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM5040QPRO/
released
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
eeec16fe-273a-44b0-b8b4-ab4d1ca14101
/measurement-sets/IGVFDS2708HTXW/
IGVFDS2708HTXW
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep1-BinA_AmpliconSequencing
PPIF
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM8646DYNI/
released
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
eaf2c3f2-0680-4c53-a3b2-5974796b9258
/measurement-sets/IGVFDS5477WNYP/
IGVFDS5477WNYP
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep3-BinD_AmpliconSequencing
PPIF
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM9885ZFLQ/
released
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
e015db4d-ce0a-439f-a221-9ad813fb9975
/measurement-sets/IGVFDS2059ELNJ/
IGVFDS2059ELNJ
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep2-BinB_AmpliconSequencing
PPIF
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM2837HWZC/
released
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
dc6751d9-4e61-4b41-962b-0b8c34a84adc
/measurement-sets/IGVFDS2890JPBI/
IGVFDS2890JPBI
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep1-BinD_AmpliconSequencing
PPIF
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM9682FICS/
released
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
d81b514b-3109-47c3-a755-bb413738a845
/measurement-sets/IGVFDS4989HPTW/
IGVFDS4989HPTW
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-THP1-PE2_Exp1_NegControl_AmpliconSequencing
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM0894QRYX/
released
untransfected prime editing Variant-EFFECTS
Homo sapiens
cb955d48-21de-490a-8627-d9a9ff25daf9
/measurement-sets/IGVFDS1144XHLC/
IGVFDS1144XHLC
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep2-BinA_AmpliconSequencing
PPIF
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM0040UFBQ/
released
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
ca947d9f-6099-4a93-81c0-62ebe0f0ec8e
/measurement-sets/IGVFDS0816EELG/
IGVFDS0816EELG
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep2-BinA_AmpliconSequencing
PPIF
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM1367KBNP/
released
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
b28c6509-1719-4d74-b014-7fa1da3af9e4
/measurement-sets/IGVFDS3823MIDB/
IGVFDS3823MIDB
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep3-FACS_Input_AmpliconSequencing
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM7107DJSZ/
released
unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
a03771eb-e2e2-427c-a3d4-e6de523b4a11
/measurement-sets/IGVFDS7933SDSN/
IGVFDS7933SDSN
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep4-BinB_AmpliconSequencing
PPIF
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM3035HZXX/
released
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
9f757a98-04d1-4835-9b18-192c1cd727cd
/measurement-sets/IGVFDS1285SAVV/
IGVFDS1285SAVV
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep3-BinC_AmpliconSequencing
PPIF
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM3275SABA/
released
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
9e03f5d5-8539-4eb2-bbef-81f030d785ff
/measurement-sets/IGVFDS7083JDYN/
IGVFDS7083JDYN
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep2-BinD_AmpliconSequencing
PPIF
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM8031DKZT/
released
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
9d3027f8-c591-45ed-b943-1d9427a81794
/measurement-sets/IGVFDS5341DFHM/
IGVFDS5341DFHM
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep3-BinB_AmpliconSequencing
PPIF
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM3794OZOG/
released
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
948acf22-b623-479d-bd7a-f1286250dcc4
/measurement-sets/IGVFDS6176JWXB/
IGVFDS6176JWXB
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep2-FACS_Input_AmpliconSequencing
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM2185RMNT/
released
unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
9259ca8d-2c3e-4c03-aa2c-74e5fb50975a
/measurement-sets/IGVFDS7952EIJE/
IGVFDS7952EIJE
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep1-BinB_AmpliconSequencing
PPIF
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM7227LRMC/
released
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
911aaffb-7a16-442f-ac4a-f3ab48d37aa0
/measurement-sets/IGVFDS8518OHPN/
IGVFDS8518OHPN
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep1-FACS_Input_AmpliconSequencing
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM4866SSGO/
released
unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
8f9f4a1b-d3e0-4a24-8614-82cd593d0102
/measurement-sets/IGVFDS2074JWFW/
IGVFDS2074JWFW
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep2-BinC_AmpliconSequencing
PPIF
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM9725CVMG/
released
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
88718d19-7aa0-4ed7-8b52-8252474c0090
/measurement-sets/IGVFDS4823GFCR/
IGVFDS4823GFCR
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep4-BinA_AmpliconSequencing
PPIF
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM7460SNVF/
released
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
75974895-0210-49d1-8d31-8b7edc7379d4
/measurement-sets/IGVFDS5892ADAN/
IGVFDS5892ADAN
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep4-FACS_Input_AmpliconSequencing
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM5918TEKW/
released
unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
6e5f92e3-4159-43f5-864c-4f8be161116c
/measurement-sets/IGVFDS0471SKEE/
IGVFDS0471SKEE
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep3-BinB_AmpliconSequencing
PPIF
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM0686AMNU/
released
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
6b3060f9-5267-4da7-b22f-d32edb551998
/measurement-sets/IGVFDS8050CYYG/
IGVFDS8050CYYG
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep3-FACS_Input_AmpliconSequencing
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO7359LVPI/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM5729BNQM/
released
unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
64287c71-9d52-4166-88c5-443471305779