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IGVFDS9477ARWI

released
File Set Type
experimental data
Summary
untransfected prime editing Variant-EFFECTS
Assay Term
in vitro CRISPR screen using flow cytometry
Sample Summaries
  • Jurkat cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)
Protocols
Aliases
jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-Jurkat-PE2_Exp1_NegControl_AmpliconSequencing
jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-Jurkat-PE2_Exp1_NegControl_AmpliconSequencing

Sequencing Results (Illumina)

8 items
fastq
reads
I2
1
GACCTAAC:GTCA
3.2 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
TTGTCTAT:ACTG
3.1 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
TTGTCTAT:ACTG
3.4 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
TTGTCTAT:ACTG
12 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
GACCTAAC:GTCA
3.4 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
GACCTAAC:GTCA
7.6 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
GACCTAAC:GTCA
12 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
TTGTCTAT:ACTG
7.5 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Jurkat cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_Jurkat
male
Homo sapiens

File Sets Using This Measurement Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
prime editing Variant-EFFECTS targeting PPIF: variant effects
  • jesse-engreitz:PPIF_promoter_8mer_insertion_Jurkat-NoStimulation_Variant-FlowFISH_analysis
Jesse Engreitz, Stanford

File Sets Controlled by This Measurement Set

98 items
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep2-FFrep1-BinA_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep3-FFrep1-BinA_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep4-FFrep2-BinD_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep4-FFrep1-BinD_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep2-FFrep2-BinA_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep3-FFrep1-FACS_Input_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep1-FFrep2-BinE_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep1-FFrep2-BinB_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep4-FFrep1-FACS_Input_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep3-FFrep2-BinC_AmpliconSequencing
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford