Home
Home
Search (⌘K or ⌃K)
Data
Methodology
Data Model
About
Help
Sign In
INDEXED
Home
/
Measurement Set
/
IGVFDS9179GDAL
IGVFDS9179GDAL
released
Json view
File Set Type
experimental data
Summary
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
cas mediated mutagenesis
Sample Summaries
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with a guide library
Construct Library Sets
IGVFDS2374NXLW
Guide (sgRNA) library targeting sequence variants in a genomic locus
Protocols
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
Aliases
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep4-BinD_AmpliconSequencing
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM5444NCSW
InVitroSystem
THP-1
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with a guide library
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO7359LVPI
igvf:donor_of_THP1
male
Homo sapiens
Sequencing Results (Illumina)
48 items
1
2
3
4
5
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI0294QFIC
fastq
reads
I1
1
Illumina NextSeq 550
TTATATCT:CAGT
605 KB
Jesse Engreitz, Stanford
validated
IGVFFI0858VWQU
fastq
reads
I2
1
Illumina NextSeq 550
TTATATCT:CAGT
567 KB
Jesse Engreitz, Stanford
validated
IGVFFI0884RFXL
fastq
reads
I2
1
Illumina NextSeq 550
TGCTGCTG:GTCA
1.6 MB
Jesse Engreitz, Stanford
validated
IGVFFI0980IGCD
fastq
reads
I2
1
Illumina NextSeq 550
TTATATCT:GTCA
1.4 MB
Jesse Engreitz, Stanford
validated
IGVFFI1171POCR
fastq
reads
I2
1
Illumina NextSeq 550
TGTAACTC:CAGT
845 KB
Jesse Engreitz, Stanford
validated
IGVFFI1217KOJK
fastq
reads
I1
1
Illumina NextSeq 550
TATCCAGG:CAGT
812 KB
Jesse Engreitz, Stanford
validated
IGVFFI1975JPXT
fastq
reads
R1
1
Illumina NextSeq 550
TTGTCTAT:GTCA
5.3 MB
Jesse Engreitz, Stanford
validated
IGVFFI2244BTOF
fastq
reads
R1
1
Illumina NextSeq 550
TGCTGCTG:GTCA
6.1 MB
Jesse Engreitz, Stanford
validated
IGVFFI2623NSIA
fastq
reads
I2
1
Illumina NextSeq 550
TGTAACTC:GTCA
1.4 MB
Jesse Engreitz, Stanford
validated
IGVFFI3000KQBI
fastq
reads
R2
1
Illumina NextSeq 550
TTGTCTAT:GTCA
6.8 MB
Jesse Engreitz, Stanford
validated
Control File Sets
1 item
Accession
Summary
Aliases
Lab
IGVFDS4989HPTW
cas mediated mutagenesis (Variant FlowFISH)
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-THP1-PE2_Exp1_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford
Attribution
Award
HG011972
Principal Investigator(s)
Anshul Kundaje
,
Jesse Engreitz
,
Marlene Rabinovitch
,
Lars Steinmetz
,
Michael Bassik
,
Thomas Quertermous
,
Will Greenleaf
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford