OBI_0003661

Ian Whaling

in vitro CRISPR screen using flow cytometry

HG011972

Stanford Center for Connecting DNA Variants to Function and Phenotype

Jesse Engreitz

jesse-engreitz

Jesse Engreitz, Stanford

Anshul Kundaje

Marlene Rabinovitch

Lars Steinmetz

Michael Bassik

Thomas Quertermous

Will Greenleaf

Guide (sgRNA) library targeting sequence variants in a genomic locus

THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 90-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

prime editing Variant FlowFISH integrating a guide (sgRNA) library targeting sequence variants in a genomic locus

THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 55-65% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 35-45% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 11-20% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 80-90% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-10% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)

IGVFDS6385JXFZ

Attribution

IGVFDS6385JXFZ

Samples

Donors

File Sets Using This Measurement Set as an Input

File Sets Controlled by This Measurement Set

Attribution