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INDEXED
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Measurement Set
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IGVFDS0634BYKS
IGVFDS0634BYKS
released
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File Set Type
experimental data
Summary
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
cas mediated mutagenesis
Sample Summaries
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 55-65% expression of PPIF) modified with a guide library
Construct Library Sets
IGVFDS6377KNCH
Guide (sgRNA) library targeting sequence variants in a genomic locus
Protocols
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
Aliases
jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep3-FFrep1-BinD_AmpliconSequencing
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM0320EORI
InVitroSystem
THP-1
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 55-65% expression of PPIF) modified with a guide library
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO7359LVPI
igvf:donor_of_THP1
male
Homo sapiens
Sequencing Results (Illumina)
8 items
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI0145LPCE
fastq
reads
R1
2
Illumina NextSeq 550
GAAGAAGT:TGAC
6.8 MB
Jesse Engreitz, Stanford
validated
IGVFFI3092JDSK
fastq
reads
I1
2
Illumina NextSeq 550
GAAGAAGT:TGAC
2.4 MB
Jesse Engreitz, Stanford
validated
IGVFFI3430EIDL
fastq
reads
R2
2
Illumina NextSeq 550
GAAGAAGT:TGAC
13 MB
Jesse Engreitz, Stanford
validated
IGVFFI3894YODL
fastq
reads
I1
2
Illumina NextSeq 550
GAAGAAGT:ACTG
2.4 MB
Jesse Engreitz, Stanford
validated
IGVFFI4637ORLS
fastq
reads
I2
2
Illumina NextSeq 550
GAAGAAGT:ACTG
2.3 MB
Jesse Engreitz, Stanford
validated
IGVFFI5732GWVX
fastq
reads
I2
2
Illumina NextSeq 550
GAAGAAGT:TGAC
2.4 MB
Jesse Engreitz, Stanford
validated
IGVFFI6059VDZF
fastq
reads
R2
2
Illumina NextSeq 550
GAAGAAGT:ACTG
13 MB
Jesse Engreitz, Stanford
validated
IGVFFI8548IENT
fastq
reads
R1
2
Illumina NextSeq 550
GAAGAAGT:ACTG
6.9 MB
Jesse Engreitz, Stanford
validated
Control File Sets
1 item
Accession
Summary
Aliases
Lab
IGVFDS6385JXFZ
cas mediated mutagenesis (Variant FlowFISH)
jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-THP1-PE2_Exp3_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford
Attribution
Award
HG011972
Principal Investigator(s)
Anshul Kundaje
,
Jesse Engreitz
,
Marlene Rabinovitch
,
Lars Steinmetz
,
Michael Bassik
,
Thomas Quertermous
,
Will Greenleaf
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford