IGVFDS0634BYKS

released
File Set Type
experimental data
Summary
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus followed by DNA sequencing assay
Assay Term
Donors
Samples
Sample Summaries
  • THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 55-65% expression of PPIF) modified with a guide library
Construct Library Sets
  • IGVFDS6377KNCH Guide (sgRNA) library targeting sequence variants in a genomic locus
Aliases
jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep3-FFrep1-BinD_AmpliconSequencing

Assay Details

Sequencing Results (Illumina)

8 items
fastq
reads
R1
2
Illumina NextSeq 550
GAAGAAGT:TGAC
6.8 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I1
2
Illumina NextSeq 550
GAAGAAGT:TGAC
2.4 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R2
2
Illumina NextSeq 550
GAAGAAGT:TGAC
13 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I1
2
Illumina NextSeq 550
GAAGAAGT:ACTG
2.4 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I2
2
Illumina NextSeq 550
GAAGAAGT:ACTG
2.3 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I2
2
Illumina NextSeq 550
GAAGAAGT:TGAC
2.4 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R2
2
Illumina NextSeq 550
GAAGAAGT:ACTG
13 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R1
2
Illumina NextSeq 550
GAAGAAGT:ACTG
6.9 MB
Jesse Engreitz, Stanford
released
validated

Control File Sets

1 item
Accession
Summary
Aliases
Lab
Status
cas mediated mutagenesis (Variant FlowFISH) followed by DNA sequencing assay
  • jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-THP1-PE2_Exp3_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford
released

Attribution