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IGVFDS5128CXIS

released
File Set Type
experimental data
Summary
untransfected prime editing Variant-EFFECTS
Assay Term
in vitro CRISPR screen using flow cytometry
Sample Summaries
  • THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)
Protocols
Aliases
jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-THP1-PE2_Exp4_NegControl_AmpliconSequencing

Sequencing Results (Illumina)

8 items
fastq
reads
I2
1
AGGTTATC:TTGT
2.1 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
GTCTGATG:TTGT
2.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
AGGTTATC:TTGT
8.6 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
GTCTGATG:TTGT
8.3 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
GTCTGATG:TTGT
10.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
AGGTTATC:TTGT
2.2 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
AGGTTATC:TTGT
10 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
GTCTGATG:TTGT
2.1 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_THP1
male
Homo sapiens

File Sets Using This Measurement Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
prime editing Variant-EFFECTS targeting PPIF: variant effects
  • jesse-engreitz:PPIF_promoter_Enformer_edits_THP1_Variant-FlowFISH_analysis
Jesse Engreitz, Stanford

File Sets Controlled by This Measurement Set

40 items
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep2-FFrep1-BinB_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep1-FFrep1-BinB_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep2-FFrep3-BinC_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep2-FFrep2-BinC_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep2-FFrep4-BinC_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep2-FFrep3-FACS_Input_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep1-FFrep1-FACS_Input_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep1-FFrep3-BinC_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep1-FFrep3-BinA_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep2-FFrep4-BinB_AmpliconSequencing
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford