IGVFDS2247EEVD

released
File Set Type
experimental data
Summary
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus followed by DNA sequencing assay
Assay Term
Donors
Samples
Sample Summaries
  • THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with a guide library
Construct Library Sets
  • IGVFDS2385DHZO Guide (sgRNA) library targeting sequence variants in a genomic locus
Aliases
jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep2-FFrep4-BinC_AmpliconSequencing

Assay Details

Sequencing Results (Illumina)

48 items
fastq
reads
R1
1
Illumina NextSeq 550
GAACCTAG:TGAC
9.2 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I1
1
Illumina NextSeq 550
CCAGTTAG:TGAC
2.1 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R1
1
Illumina NextSeq 550
GCCGCAAC:TGAC
8.4 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R2
1
Illumina NextSeq 550
GAACCTAG:TGAC
12 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I2
1
Illumina NextSeq 550
GTCGCCTT:TGAC
2.0 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I2
1
Illumina NextSeq 550
CCTACCAT:TGAC
1.9 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I2
1
Illumina NextSeq 550
GAACCTAG:TGAC
2.0 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R1
1
Illumina NextSeq 550
GCCTAGCC:TGAC
9.2 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I2
1
Illumina NextSeq 550
GCCGCAAC:TGAC
1.8 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R2
1
Illumina NextSeq 550
GATATCCA:TGAC
11 MB
Jesse Engreitz, Stanford
released
validated

Control File Sets

1 item
Accession
Summary
Aliases
Lab
Status
cas mediated mutagenesis (Variant FlowFISH) followed by DNA sequencing assay
  • jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-THP1-PE2_Exp4_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford
released

Attribution