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INDEXED
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Measurement Set
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IGVFDS2247EEVD
IGVFDS2247EEVD
released
Json view
File Set Type
experimental data
Summary
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
cas mediated mutagenesis
Sample Summaries
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with a guide library
Construct Library Sets
IGVFDS2385DHZO
Guide (sgRNA) library targeting sequence variants in a genomic locus
Protocols
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
Aliases
jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep2-FFrep4-BinC_AmpliconSequencing
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM1406ICND
InVitroSystem
THP-1
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with a guide library
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO7359LVPI
igvf:donor_of_THP1
male
Homo sapiens
Sequencing Results (Illumina)
48 items
1
2
3
4
5
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI0253MCNS
fastq
reads
R1
1
Illumina NextSeq 550
GAACCTAG:TGAC
9.2 MB
Jesse Engreitz, Stanford
validated
IGVFFI0405WSUF
fastq
reads
I1
1
Illumina NextSeq 550
CCAGTTAG:TGAC
2.1 MB
Jesse Engreitz, Stanford
validated
IGVFFI0407QZNJ
fastq
reads
R1
1
Illumina NextSeq 550
GCCGCAAC:TGAC
8.4 MB
Jesse Engreitz, Stanford
validated
IGVFFI0544QMJM
fastq
reads
R2
1
Illumina NextSeq 550
GAACCTAG:TGAC
12 MB
Jesse Engreitz, Stanford
validated
IGVFFI0804NXWR
fastq
reads
I2
1
Illumina NextSeq 550
GTCGCCTT:TGAC
2.0 MB
Jesse Engreitz, Stanford
validated
IGVFFI0909DKEX
fastq
reads
I2
1
Illumina NextSeq 550
CCTACCAT:TGAC
1.9 MB
Jesse Engreitz, Stanford
validated
IGVFFI1008ASKE
fastq
reads
I2
1
Illumina NextSeq 550
GAACCTAG:TGAC
2.0 MB
Jesse Engreitz, Stanford
validated
IGVFFI1215XWXM
fastq
reads
R1
1
Illumina NextSeq 550
GCCTAGCC:TGAC
9.2 MB
Jesse Engreitz, Stanford
validated
IGVFFI1276KXJX
fastq
reads
I2
1
Illumina NextSeq 550
GCCGCAAC:TGAC
1.8 MB
Jesse Engreitz, Stanford
validated
IGVFFI1336UKJI
fastq
reads
R2
1
Illumina NextSeq 550
GATATCCA:TGAC
11 MB
Jesse Engreitz, Stanford
validated
Control File Sets
1 item
Accession
Summary
Aliases
Lab
IGVFDS5128CXIS
cas mediated mutagenesis (Variant FlowFISH)
jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-THP1-PE2_Exp4_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford
Attribution
Award
HG011972
Principal Investigator(s)
Anshul Kundaje
,
Jesse Engreitz
,
Marlene Rabinovitch
,
Lars Steinmetz
,
Michael Bassik
,
Thomas Quertermous
,
Will Greenleaf
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford