IGVFDS3030YDAT

released
File Set Type
experimental data
Summary
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus followed by DNA sequencing assay
Assay Term
Donors
Samples
Sample Summaries
  • Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 55-65% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with a guide library
Construct Library Sets
  • IGVFDS6377KNCH Guide (sgRNA) library targeting sequence variants in a genomic locus
Aliases
jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep3-FFrep2-BinD_AmpliconSequencing

Assay Details

Sequencing Results (Illumina)

8 items
fastq
reads
I1
1
Illumina NextSeq 550
ATTATGTT:GTCA
3.3 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I2
1
Illumina NextSeq 550
ATTATGTT:GTCA
3.1 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R2
1
Illumina NextSeq 550
CCTACCAT:ACTG
15 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I2
1
Illumina NextSeq 550
CCTACCAT:ACTG
3.3 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
I1
1
Illumina NextSeq 550
CCTACCAT:ACTG
3.4 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R2
1
Illumina NextSeq 550
ATTATGTT:GTCA
14 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R1
1
Illumina NextSeq 550
ATTATGTT:GTCA
7.4 MB
Jesse Engreitz, Stanford
released
validated
fastq
reads
R1
1
Illumina NextSeq 550
CCTACCAT:ACTG
7.8 MB
Jesse Engreitz, Stanford
released
validated

Control File Sets

1 item
Accession
Summary
Aliases
Lab
Status
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus followed by DNA sequencing assay
  • jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-Jurkat-PE2_Exp1_NegControl_AmpliconSequencing
  • jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-Jurkat-PE2_Exp1_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford
released

Attribution