Jurkat — IGVFSM4844JJJX

released
Summary
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 55-65% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with a guide library
Sample Terms
Sex
male
Age
unknown
Donors
Taxa
Homo sapiens
Sources
Sorted From Sample
IGVFSM3716YNOG FACS bin for 55-65% expression of PPIF
Aliases
jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep3-FFrep2-BinD

File Sets

2 items
primary analysis of Variant FlowFISH data
  • jesse-engreitz:PPIF_promoter_8mer_insertion_Jurkat-Stimulation_Variant-FlowFISH_analysis
Jesse Engreitz, Stanford
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus followed by DNA sequencing assay
  • jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep3-FFrep2-BinD_AmpliconSequencing
Jesse Engreitz, Stanford

Modifications

1 item

Treatments

2 items
Summary
stimulation
chemical
Treatment of 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours
stimulation
protein
Treatment of 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours

Attribution