OBI_0003661

Ian Whaling

in vitro CRISPR screen using flow cytometry

HG011972

Stanford Center for Connecting DNA Variants to Function and Phenotype

Jesse Engreitz

jesse-engreitz

Jesse Engreitz, Stanford

Anshul Kundaje

Marlene Rabinovitch

Lars Steinmetz

Michael Bassik

Thomas Quertermous

Will Greenleaf

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 0-10% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with a guide library

Jurkat cell line, male, Homo sapiens treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with a guide library

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 11-20% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with a guide library

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 35-45% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with a guide library

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 55-65% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with a guide library

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 80-90% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with a guide library

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 90-100% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with a guide library

principal analysis of Variant FlowFISH data

audit_item_status

audit_inconsistent_sequencing_kit

audit_missing_seqspec

audit_missing_auxiliary_set

audit_input_file_set_for

Variant FlowFISH

Guide (sgRNA) library targeting sequence variants in a genomic locus

Treatment of 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours

Treatment of 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours

Michael Montgomery

prime editing Variant FlowFISH integrating a guide (sgRNA) library targeting sequence variants in a genomic locus

IGVFDS3030YDAT

Attribution

IGVFDS3030YDAT

Samples

Donors

Control File Sets

File Sets Using This Measurement Set as an Input

Attribution