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Analysis of PPIF promoter 5-bp tiling mutagenesis edit effects on PPIF expression that were measured by Variant-FlowFISH in THP-1 cells.

THP-1 cell line, modified with a guide library, sorted on expression of PPIF

THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with a guide library

THP-1 cell line, male, Homo sapiens modified with a guide library

THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of PPIF) modified with a guide library

THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with a guide library

THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with a guide library

principal analysis of Variant FlowFISH data

Guide (sgRNA) library targeting sequence variants in a genomic locus

prime editing Variant FlowFISH integrating a guide (sgRNA) library targeting sequence variants in a genomic locus