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INDEXED
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Measurement Set
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IGVFDS8356GTHO
IGVFDS8356GTHO
released
Json view
File Set Type
experimental data
Summary
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
cas mediated mutagenesis
Sample Summaries
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with a guide library
Construct Library Sets
IGVFDS6982DESN
Guide (sgRNA) library targeting sequence variants in a genomic locus
Protocols
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
Aliases
jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep1-BinD_AmpliconSequencing
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM9715TWHE
InVitroSystem
THP-1
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with a guide library
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO7359LVPI
igvf:donor_of_THP1
male
Homo sapiens
Sequencing Results (Illumina)
48 items
1
2
3
4
5
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI0046QKFS
fastq
reads
R1
1
Illumina NextSeq 550
TGCTCGAC:GTCA
7.6 MB
Jesse Engreitz, Stanford
validated
IGVFFI0142NJLL
fastq
reads
I2
1
Illumina NextSeq 550
TATCTGCC:GTCA
2.2 MB
Jesse Engreitz, Stanford
validated
IGVFFI0195KDJP
fastq
reads
R1
1
Illumina NextSeq 550
TAAGCACA:GTCA
7.7 MB
Jesse Engreitz, Stanford
validated
IGVFFI0265AZWF
fastq
reads
I1
1
Illumina NextSeq 550
TAAGCACA:GTCA
2.7 MB
Jesse Engreitz, Stanford
validated
IGVFFI0467ULNZ
fastq
reads
R2
1
Illumina NextSeq 550
TCGCTAGA:GTCA
10 MB
Jesse Engreitz, Stanford
validated
IGVFFI0669UVXO
fastq
reads
R1
1
Illumina NextSeq 550
TGTAATCA:GTCA
7.5 MB
Jesse Engreitz, Stanford
validated
IGVFFI0925HJEU
fastq
reads
I2
1
Illumina NextSeq 550
TTCGCTGA:GTCA
1.7 MB
Jesse Engreitz, Stanford
validated
IGVFFI0934INHF
fastq
reads
R2
1
Illumina NextSeq 550
TAAGCACA:GTCA
11 MB
Jesse Engreitz, Stanford
validated
IGVFFI0959UBMT
fastq
reads
I1
1
Illumina NextSeq 550
TTGAATAG:GTCA
2.5 MB
Jesse Engreitz, Stanford
validated
IGVFFI1148TMWC
fastq
reads
I2
1
Illumina NextSeq 550
TAAGCACA:GTCA
2.3 MB
Jesse Engreitz, Stanford
validated
Control File Sets
1 item
Accession
Summary
Aliases
Lab
IGVFDS5864UGFT
cas mediated mutagenesis (Variant FlowFISH)
jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-THP1-PE2_Exp2_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford
Attribution
Award
HG011972
Principal Investigator(s)
Anshul Kundaje
,
Jesse Engreitz
,
Marlene Rabinovitch
,
Lars Steinmetz
,
Michael Bassik
,
Thomas Quertermous
,
Will Greenleaf
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford