HG011966

Ian Whaling

Massively parallel characterization of variants and elements impacting transcriptional regulation in dynamic cellular systems

Jay Shendure

jay-shendure

Jay Shendure, UW

Nadav Ahituv

Martin Kircher

K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library

gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci

K562 cell line (cellular sub pool: 10Xlane1), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library

scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci

K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library

K562 cell line (cellular sub pool: 10Xlane1), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library

File format specification for SPECTRE tables

IGVFFI4612KEHH

differential gene expression quantifications

SCEPTRE K562 hits (FDR < 0.1). Two-tailed SCEPTRE P-values were derived using default parameters. SCEPTRE P-values were then Benjamini-Hochberg corrected and those < 0.1 were kept for two-sided discovery sets.

scCRISPR activation screen: differential gene expression quantifications, filtered feature barcode matrix

IGVFFI4612KEHH

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