Home

IGVFFI3289WICA

released
validated
File Set
IGVFDS9143TFRW(CRISPR interference FACS screen targeting CCR7 integrating a guide (sgRNA) library targeting transcription start sites in many genes with low FACS signal control)
Summary
custom assembly guide quantifications
Analysis Step Version
/analysis-step-versions/886b365e-3105-424a-8972-7476238b54d7/
File Format
csv
Content Type
guide quantifications
Aliases
charles-gersbach:cd8-tcells-guide-counts-crispri-ccr7
md5sum
3d11ec02c679a13b3bc7f61da0ecc7cb
Content MD5sum
903e8e9efa20a31850a542e0b65849e7
File Size
40 KB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/CRISPRi.TF.CCR7.counts.table.csv.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Charles Gersbach, Duke

Reference Source Details

Genome Assembly
custom

File Format Specifications

1 item
Page
Description
Type
Download
Standardized File Format
Preview
gRNA counts table
file format specification

Samples

4 items
Sample Terms
Summary
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)

Files This File Derives From

4 items
bam
alignments
Charles Gersbach, Duke
56 MB
validated
bam
alignments
Charles Gersbach, Duke
36 MB
validated
bam
alignments
Charles Gersbach, Duke
14 MB
validated
bam
alignments
Charles Gersbach, Duke
9.5 MB
validated

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
fold change over control
custom assembly fold change over control
Charles Gersbach, Duke
70 KB
validated