{
"@context": "/terms/",
"@id": "/tabular-files/IGVFFI3289WICA/",
"@type": [
"TabularFile",
"File",
"Item"
],
"accession": "IGVFFI3289WICA",
"aliases": [
"charles-gersbach:cd8-tcells-guide-counts-crispri-ccr7"
],
"analysis_step_version": "/analysis-step-versions/886b365e-3105-424a-8972-7476238b54d7/",
"assay_titles": [
"CRISPR FACS screen"
],
"assembly": "custom",
"audit": {},
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization"
},
"barcode_map_for": [],
"content_md5sum": "903e8e9efa20a31850a542e0b65849e7",
"content_type": "guide quantifications",
"controlled_access": false,
"creation_timestamp": "2024-09-20T18:39:23.858127+00:00",
"derived_from": [
"/alignment-files/IGVFFI6752WOMF/",
"/alignment-files/IGVFFI8745XXNE/",
"/alignment-files/IGVFFI7658XOUY/",
"/alignment-files/IGVFFI1939MBPM/"
],
"file_format": "csv",
"file_format_specifications": [
"/documents/7f185ed1-f80f-470c-9a5f-50fdd114d9a6/"
],
"file_set": {
"@id": "/analysis-sets/IGVFDS9143TFRW/",
"accession": "IGVFDS9143TFRW",
"file_set_type": "principal analysis",
"samples": [
{
"@id": "/primary-cells/IGVFSM1205VMNO/",
"accession": "IGVFSM1205VMNO",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM7413JCSK/",
"accession": "IGVFSM7413JCSK",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM2207SJEN/",
"accession": "IGVFSM2207SJEN",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM9398LTWW/",
"accession": "IGVFSM9398LTWW",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
}
],
"summary": "CRISPR interference FACS screen targeting CCR7: fold change over control, guide quantifications"
},
"file_size": 39697,
"gene_list_for": [],
"href": "/tabular-files/IGVFFI3289WICA/@@download/IGVFFI3289WICA.csv.gz",
"input_file_for": [
"/tabular-files/IGVFFI0420XBKB/"
],
"integrated_in": [],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"loci_list_for": [],
"md5sum": "3d11ec02c679a13b3bc7f61da0ecc7cb",
"release_timestamp": "2024-11-18T21:09:09.496027+00:00",
"s3_uri": "s3://igvf-files/2024/09/20/d644badc-4435-4e61-aae5-a47a86399c3b/IGVFFI3289WICA.tsv.gz",
"schema_version": "13",
"status": "released",
"submitted_by": {
"@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
"title": "Alejandro Barrera"
},
"submitted_file_name": "/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/CRISPRi.TF.CCR7.counts.table.csv.gz",
"summary": "custom assembly guide quantifications",
"upload_status": "validated",
"uuid": "d644badc-4435-4e61-aae5-a47a86399c3b"
}