IGVFFI2629EIOZ

released

validated

File Set

IGVFDS8364HJHL(CRISPR knockout FACS screen targeting IL7R integrating an expression vector library of BATF3 and a guide (sgRNA) library targeting transcription start sites in many genes with low FACS signal control)

Summary

custom assembly fold change over control

Analysis Step Version

/analysis-step-versions/6a1d20c7-c92f-47d6-8e3b-5c74b3e8eed2/

File Format

tsv

Content Type

fold change over control

Aliases

charles-gersbach:cd8-tcells-guide-quantifications-crispr-ko-tfome-batf3-over

md5sum

4075ce4fb083ec2cff3e93d3acd235d8

Content MD5sum

51ba6013fa2e71b5851c42ebd5c1a69d

File Size

376 KB

Submitted File Name

/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/T6B_DESeq2_TFko_with_BATF3.simple.v2.txt.gz

Checkfiles Version

Legacy version of checkfiles

Attribution

Charles Gersbach, Duke

(

HG012053 — Charles Gersbach

)

Reference Source Details

Genome Assembly

custom

File Format Specifications

1 item

Page

Description

Type

Download

Standardized File Format

Preview

DESeq2 results

file format specification

DESeq2 results file format specification.pdf

Samples

4 items

Sample Terms

Summary

IGVFSM3895SURE

PrimaryCell

CD8-positive, alpha-beta memory T cell

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)

IGVFSM7158ADKU

PrimaryCell

CD8-positive, alpha-beta memory T cell

IGVFSM7887TFLH

PrimaryCell

CD8-positive, alpha-beta memory T cell

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)

IGVFSM8084QKPW

PrimaryCell

CD8-positive, alpha-beta memory T cell