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IGVFDS8364HJHL

released
Aliases
charles-gersbach:cd8-tcells-analysis-set-crispr-ko-tfome-batf3-over
File Set Type
principal analysis
Summary
CRISPR knockout FACS screen targeting IL7R: alignments, fold change over control, guide quantifications
Workflows
Publications
Samples
CD8-positive, alpha-beta memory T primary cell primary cell, modified with CRISPRko Sp-Cas9, transfected with a guide library, expression vector library, sorted on expression of IL7R
Protocols

Files

6 items
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
446 MB
validated
tsv
fold change over control
custom assembly fold change over control
Charles Gersbach, Duke
376 KB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
473 MB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
437 MB
validated
csv
guide quantifications
custom assembly guide quantifications
Charles Gersbach, Duke
144 KB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
380 MB
validated

File Association Graph

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File Set
File
AnalysisSet
MeasurementSet

Samples

4 items
Sample Terms
Associated Construct Library Sets
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)

Donors

2 items
Aliases
  • charles-gersbach:cd8-tcells-donor-26
unspecified
Homo sapiens
  • charles-gersbach:cd8-tcells-donor-25
unspecified
Homo sapiens

Associated Construct Library Sets

2 items
Summary
Library Design Files
expression vector library of BATF3
guide (sgRNA) library targeting transcription start sites in many genes

MeasurementSet Input File Sets

4 items
Controls
CRISPR knockout FACS screen sorted on the expression of IL7R integrating a guide (sgRNA) library targeting transcription start sites in many genes and an expression vector library of BATF3
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)
CRISPR knockout FACS screen sorted on the expression of IL7R integrating an expression vector library of BATF3 and a guide (sgRNA) library targeting transcription start sites in many genes
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)
low FACS signal CRISPR knockout FACS screen sorted on the expression of IL7R integrating a guide (sgRNA) library targeting transcription start sites in many genes and an expression vector library of BATF3
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)
low FACS signal CRISPR knockout FACS screen sorted on the expression of IL7R integrating an expression vector library of BATF3 and a guide (sgRNA) library targeting transcription start sites in many genes
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke