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IGVFFI2207HLUJ

released
validated
File Set
IGVFDS2261TIMI(CRISPR activation FACS screen targeting IL2RA integrating a guide (sgRNA) library targeting transcription start sites in IL2RA with low FACS signal control)
Summary
custom assembly fold change over control
Analysis Step Version
/analysis-step-versions/6a1d20c7-c92f-47d6-8e3b-5c74b3e8eed2/
File Format
tsv
Content Type
fold change over control
Aliases
charles-gersbach:jurkats-guide-quantifications-crispra-vp64-il2ra
md5sum
c657eba469c8190ce7d70e7fe05d9214
Content MD5sum
16a2be7bb6b739f27ea452b2c301f047
File Size
14 KB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/T1C_IL2RA_CRISPRa1xVP64_screen.simple.v2.txt.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Charles Gersbach, Duke

Reference Source Details

Genome Assembly
custom

File Format Specifications

1 item
Page
Description
Type
Download
Standardized File Format
Preview
DESeq2 results
file format specification

Samples

6 items
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Files This File Derives From

1 item
Accession
File Set
File Format
Content Type
Lab
File Size
Upload Status
csv
guide quantifications
Charles Gersbach, Duke
9.4 KB
validated