IGVFFI2207HLUJ

released
validated
{
    "@context": "/terms/",
    "@id": "/tabular-files/IGVFFI2207HLUJ/",
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    "accession": "IGVFFI2207HLUJ",
    "aliases": [
        "charles-gersbach:jurkats-guide-quantifications-crispra-vp64-il2ra"
    ],
    "analysis_step_version": "/analysis-step-versions/6a1d20c7-c92f-47d6-8e3b-5c74b3e8eed2/",
    "assay_titles": [
        "CRISPR FACS screen"
    ],
    "assembly": "custom",
    "audit": {},
    "award": {
        "@id": "/awards/HG012053/",
        "component": "functional characterization"
    },
    "barcode_map_for": [],
    "content_md5sum": "16a2be7bb6b739f27ea452b2c301f047",
    "content_type": "fold change over control",
    "controlled_access": false,
    "creation_timestamp": "2024-09-05T20:41:01.036658+00:00",
    "derived_from": [
        "/tabular-files/IGVFFI7694LAUN/"
    ],
    "file_format": "tsv",
    "file_format_specifications": [
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    ],
    "file_set": {
        "@id": "/analysis-sets/IGVFDS2261TIMI/",
        "accession": "IGVFDS2261TIMI",
        "file_set_type": "principal analysis",
        "samples": [
            {
                "@id": "/in-vitro-systems/IGVFSM3148FRBY/",
                "accession": "IGVFSM3148FRBY",
                "classifications": [
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                ],
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                        "@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
                        "modality": "activation",
                        "status": "released",
                        "summary": "CRISPRa Sa-dCas9-VP64"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/EFO_0002796/",
                        "status": "released",
                        "term_name": "Jurkat"
                    }
                ],
                "status": "released",
                "summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/in-vitro-systems/IGVFSM1480DJBL/",
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                        "modality": "activation",
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                        "summary": "CRISPRa Sa-dCas9-VP64"
                    }
                ],
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                        "@id": "/sample-terms/EFO_0002796/",
                        "status": "released",
                        "term_name": "Jurkat"
                    }
                ],
                "status": "released",
                "summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/in-vitro-systems/IGVFSM7748IVLZ/",
                "accession": "IGVFSM7748IVLZ",
                "classifications": [
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                        "modality": "activation",
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                        "summary": "CRISPRa Sa-dCas9-VP64"
                    }
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                        "@id": "/sample-terms/EFO_0002796/",
                        "status": "released",
                        "term_name": "Jurkat"
                    }
                ],
                "status": "released",
                "summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/in-vitro-systems/IGVFSM3606VJWU/",
                "accession": "IGVFSM3606VJWU",
                "classifications": [
                    "cell line"
                ],
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                    {
                        "@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
                        "modality": "activation",
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                        "summary": "CRISPRa Sa-dCas9-VP64"
                    }
                ],
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                        "@id": "/sample-terms/EFO_0002796/",
                        "status": "released",
                        "term_name": "Jurkat"
                    }
                ],
                "status": "released",
                "summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
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                "@id": "/in-vitro-systems/IGVFSM4401YRWQ/",
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                        "@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
                        "modality": "activation",
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                        "summary": "CRISPRa Sa-dCas9-VP64"
                    }
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                        "@id": "/sample-terms/EFO_0002796/",
                        "status": "released",
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                    }
                ],
                "status": "released",
                "summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/in-vitro-systems/IGVFSM2737ATVK/",
                "accession": "IGVFSM2737ATVK",
                "classifications": [
                    "cell line"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
                        "modality": "activation",
                        "status": "released",
                        "summary": "CRISPRa Sa-dCas9-VP64"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/EFO_0002796/",
                        "status": "released",
                        "term_name": "Jurkat"
                    }
                ],
                "status": "released",
                "summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            }
        ],
        "status": "released",
        "summary": "CRISPR activation FACS screen targeting IL2RA: fold change over control, guide quantifications"
    },
    "file_size": 13760,
    "gene_list_for": [],
    "href": "/tabular-files/IGVFFI2207HLUJ/@@download/IGVFFI2207HLUJ.tsv.gz",
    "input_file_for": [],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/charles-gersbach/",
        "title": "Charles Gersbach, Duke"
    },
    "loci_list_for": [],
    "md5sum": "c657eba469c8190ce7d70e7fe05d9214",
    "release_timestamp": "2024-11-18T21:09:18.393805+00:00",
    "s3_uri": "s3://igvf-files/2024/09/05/9df28753-7e21-410f-a84c-013b23eda694/IGVFFI2207HLUJ.tsv.gz",
    "schema_version": "13",
    "status": "released",
    "submitted_by": {
        "@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
        "title": "Alejandro Barrera"
    },
    "submitted_file_name": "/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/T1C_IL2RA_CRISPRa1xVP64_screen.simple.v2.txt.gz",
    "summary": "custom assembly fold change over control",
    "upload_status": "validated",
    "uuid": "9df28753-7e21-410f-a84c-013b23eda694"
}