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IGVFFI8750UNIJ

released
validated
File Set
IGVFDS8866USWP(gRNA sequencing for CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in CD2)
Summary
R1 reads from sequencing run 2
File Format
fastq
Content Type
reads
Aliases
charles-gersbach:cd8-tcells-measurement-set-crispri-cd2-high-bin-rep2-R1
md5sum
63f597abb2157ee0c149aa1dc278616b
Content MD5sum
6206ef89aa82876fbd32410453688523
File Size
29 MB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/pSM8-High-R2_S14_L001_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina MiSeq
Sequencing Run
2
Flowcell ID
000000000-CY6V2
Lane
1
Sequencing Kit
MiSeq Reagent Kit v2
Illumina Read Type
R1
Average Read Length
22
Read Count
1300346

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CD2 ) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
21 MB
validated

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke