{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI8750UNIJ/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI8750UNIJ",
"aliases": [
"charles-gersbach:cd8-tcells-measurement-set-crispri-cd2-high-bin-rep2-R1"
],
"assay_titles": [
"CRISPR FACS screen"
],
"audit": {},
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization"
},
"content_md5sum": "6206ef89aa82876fbd32410453688523",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-11-08T20:27:57.053687+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
"@id": "/auxiliary-sets/IGVFDS8866USWP/",
"accession": "IGVFDS8866USWP",
"file_set_type": "gRNA sequencing",
"samples": [
{
"@id": "/primary-cells/IGVFSM7658WDNM/",
"accession": "IGVFSM7658WDNM",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CD2 ) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
}
],
"summary": "gRNA sequencing for CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in CD2"
},
"file_size": 29355991,
"flowcell_id": "000000000-CY6V2",
"gene_list_for": [],
"href": "/sequence-files/IGVFFI8750UNIJ/@@download/IGVFFI8750UNIJ.fastq.gz",
"illumina_read_type": "R1",
"input_file_for": [
"/alignment-files/IGVFFI2317FRUV/"
],
"integrated_in": [],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"lane": 1,
"loci_list_for": [],
"maximum_read_length": 22,
"md5sum": "63f597abb2157ee0c149aa1dc278616b",
"mean_read_length": 22,
"minimum_read_length": 22,
"read_count": 1300346,
"release_timestamp": "2024-11-18T21:09:03.526281+00:00",
"s3_uri": "s3://igvf-files/2023/11/08/d0bfa0a9-78cd-442b-bc93-8b9859ae7b63/IGVFFI8750UNIJ.fastq.gz",
"schema_version": "14",
"seqspecs": [],
"sequencing_kit": "MiSeq Reagent Kit v2",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0004205/",
"term_name": "Illumina MiSeq"
},
"sequencing_run": 2,
"status": "released",
"submitted_by": {
"@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
"title": "Alejandro Barrera"
},
"submitted_file_name": "/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/pSM8-High-R2_S14_L001_R1_001.fastq.gz",
"summary": "R1 reads from sequencing run 2",
"upload_status": "validated",
"uuid": "d0bfa0a9-78cd-442b-bc93-8b9859ae7b63"
}