IGVFFI7799MHFQ

released
validated
File Set
IGVFDS2625BWKZ(gRNA sequencing for CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in B2M)
Summary
R1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
charles-gersbach:cd8-tcells-measurement-set-crispri-b2m-high-bin-rep1-R1
md5sum
6d65f219d501e81adbe5d38b01e21f2a
Content MD5sum
c28e19962708c64adadebdae7d05d9b3
File Size
20 MB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/B2M-High-R1_S5_L001_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Flowcell ID
000000000-J9HPF
Lane
1
Sequencing Kit
MiSeq Reagent Kit v2
Illumina Read Type
R1
Average Read Length
22
Read Count
843201

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
14 MB
validated

Attribution