IGVFFI7799MHFQ

{
    "@context": "/terms/",
    "@id": "/sequence-files/IGVFFI7799MHFQ/",
    "@type": [
        "SequenceFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI7799MHFQ",
    "aliases": [
        "charles-gersbach:cd8-tcells-measurement-set-crispri-b2m-high-bin-rep1-R1"
    ],
    "assay_titles": [
        "CRISPR FACS screen"
    ],
    "audit": {},
    "award": {
        "@id": "/awards/HG012053/",
        "component": "functional characterization"
    },
    "content_md5sum": "c28e19962708c64adadebdae7d05d9b3",
    "content_type": "reads",
    "controlled_access": false,
    "creation_timestamp": "2023-11-08T20:28:15.533066+00:00",
    "externally_hosted": false,
    "file_format": "fastq",
    "file_set": {
        "@id": "/auxiliary-sets/IGVFDS2625BWKZ/",
        "accession": "IGVFDS2625BWKZ",
        "file_set_type": "gRNA sequencing",
        "samples": [
            {
                "@id": "/primary-cells/IGVFSM6062UNTJ/",
                "accession": "IGVFSM6062UNTJ",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
                        "modality": "interference",
                        "status": "released",
                        "summary": "CRISPRi Sa-dCas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            }
        ],
        "status": "released",
        "summary": "gRNA sequencing for CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in B2M"
    },
    "file_size": 20299372,
    "flowcell_id": "000000000-J9HPF",
    "gene_list_for": [],
    "href": "/sequence-files/IGVFFI7799MHFQ/@@download/IGVFFI7799MHFQ.fastq.gz",
    "illumina_read_type": "R1",
    "input_file_for": [
        "/alignment-files/IGVFFI0916WHTV/"
    ],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/charles-gersbach/",
        "title": "Charles Gersbach, Duke"
    },
    "lane": 1,
    "loci_list_for": [],
    "maximum_read_length": 22,
    "md5sum": "6d65f219d501e81adbe5d38b01e21f2a",
    "mean_read_length": 22,
    "minimum_read_length": 22,
    "read_count": 843201,
    "release_timestamp": "2024-11-18T21:09:14.718540+00:00",
    "s3_uri": "s3://igvf-files/2023/11/08/cdd8d260-85f8-493f-b8a6-4b6a3c58fd11/IGVFFI7799MHFQ.fastq.gz",
    "schema_version": "14",
    "seqspecs": [],
    "sequencing_kit": "MiSeq Reagent Kit v2",
    "sequencing_platform": {
        "@id": "/platform-terms/EFO_0004205/",
        "status": "released",
        "term_name": "Illumina MiSeq"
    },
    "sequencing_run": 1,
    "status": "released",
    "submitted_by": {
        "@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
        "title": "Alejandro Barrera"
    },
    "submitted_file_name": "/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/B2M-High-R1_S5_L001_R1_001.fastq.gz",
    "summary": "R1 reads from sequencing run 1",
    "upload_status": "validated",
    "uuid": "cdd8d260-85f8-493f-b8a6-4b6a3c58fd11"
}