IGVFFI6538JYJJ

released

validated

File Set

IGVFDS2599ESDV(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin6hrs-FF1-BinA-PCR3-Rep1_S187

md5sum

8e02f06a2cee4d14b8d02d97bed2b700

Content MD5sum

36181375f6846662ce8254c21e6cb04a

File Size

33 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin6hrs-FF1-BinA-PCR3-Rep1_S187_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AAGACACT:ATTG

Average Read Length

Read Count

1541750

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM2428RDRI

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library