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IGVFFI6523BPLR

released
validated
File Set
IGVFDS8356GTHO(CRISPR prime editing Variant-EFFECTS sorted on the expression of PPIF integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep1-BinD-PCRrep9_I2
md5sum
0e509cc3e5e3170c84cc7517cd0d9d91
Content MD5sum
dd97b415de0e76fa33101a021400e582
File Size
2.3 MB
Submitted File Name
230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep1-BinD-PCRrep9_S45_I2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
I2
Index
TGTAATCA:GTCA
Average Read Length
4
Read Count
284081

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford