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IGVFFI6041TETM

released
validated
File Set
IGVFDS8749RHTV(gRNA sequencing for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus)
Summary
R1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:sequence_data_eRZ10_teloHAEC_TLNRD1_enhancer_CRISPRi_FlowFISH_Rep4_bin4
md5sum
7d8ea591827e24fac5dc3645876f2043
Content MD5sum
52d3882080726f57ac888fc6320b705c
File Size
85 MB
Submitted File Name
/Users/ronghao/Desktop/Research/data/eRZ10_FlowFISH/TLNRD1/eRZ10_FlowFISH_fastq/T4_bin4_S34_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
R1
Average Read Length
25
Read Count
3657028

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin for 76-100% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford