IGVFFI5196VSKP

released
validated
File Set
IGVFDS6052SPEY(gRNA sequencing for CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA)
Summary
R1 reads from sequencing run 2
File Format
fastq
Content Type
reads
Aliases
charles-gersbach:jurkats-measurement-set-crispra-vp64x2-il2ra-high-bin-rep2-R1
md5sum
84b04631841231ba249ac757ace4d046
Content MD5sum
71afb2cda766c48433e84bed9e477d59
File Size
28 MB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/pSM26-High-R2_S4_L001_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
2
Flowcell ID
000000000-JFKPJ
Lane
1
Sequencing Kit
MiSeq Reagent Kit v2
Illumina Read Type
R1
Average Read Length
22
Read Count
1309159

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
20 MB
validated

Attribution