{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI5196VSKP/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI5196VSKP",
"aliases": [
"charles-gersbach:jurkats-measurement-set-crispra-vp64x2-il2ra-high-bin-rep2-R1"
],
"assay_titles": [
"CRISPR FACS screen"
],
"audit": {},
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization"
},
"content_md5sum": "71afb2cda766c48433e84bed9e477d59",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-11-08T20:35:34.995447+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
"@id": "/auxiliary-sets/IGVFDS6052SPEY/",
"accession": "IGVFDS6052SPEY",
"file_set_type": "gRNA sequencing",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM6964ODEE/",
"accession": "IGVFSM6964ODEE",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/4c532ce1-11aa-41be-9757-13923c19248b/",
"modality": "activation",
"status": "released",
"summary": "CRISPRa Sa-dCas9-2xVP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"status": "released",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
}
],
"status": "released",
"summary": "gRNA sequencing for CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA"
},
"file_size": 27529680,
"flowcell_id": "000000000-JFKPJ",
"gene_list_for": [],
"href": "/sequence-files/IGVFFI5196VSKP/@@download/IGVFFI5196VSKP.fastq.gz",
"illumina_read_type": "R1",
"input_file_for": [
"/alignment-files/IGVFFI3711CGQN/"
],
"integrated_in": [],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"lane": 1,
"loci_list_for": [],
"maximum_read_length": 22,
"md5sum": "84b04631841231ba249ac757ace4d046",
"mean_read_length": 22,
"minimum_read_length": 22,
"read_count": 1309159,
"release_timestamp": "2024-11-18T21:08:59.494889+00:00",
"s3_uri": "s3://igvf-files/2023/11/08/5ae38dcf-d09c-4a18-8f44-6e2d4ac69c9b/IGVFFI5196VSKP.fastq.gz",
"schema_version": "14",
"seqspecs": [],
"sequencing_kit": "MiSeq Reagent Kit v2",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0004205/",
"status": "released",
"term_name": "Illumina MiSeq"
},
"sequencing_run": 2,
"status": "released",
"submitted_by": {
"@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
"title": "Alejandro Barrera"
},
"submitted_file_name": "/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/pSM26-High-R2_S4_L001_R1_001.fastq.gz",
"summary": "R1 reads from sequencing run 2",
"upload_status": "validated",
"uuid": "5ae38dcf-d09c-4a18-8f44-6e2d4ac69c9b"
}