IGVFFI4071NQGH

preview

validated

This object is in preview status. Metadata is subject to change until this object is released.

File Set

IGVFDS2419JBTK(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)

Summary

I2 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep1-BinB-PCRRep1_I2

md5sum

3b1403a9ae72d5a393dfeabd45ff38a2

Content MD5sum

773e51763dbd9095b47501e7f20d32db

File Size

1.5 MB

Submitted File Name

Jurkat_Stim-IL2RA_prom-BioRep1-FFRep1-PCRRep1-BinB_S2_I2_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NovaSeq X

Sequencing Run

Illumina Read Type

Index

ACCAACTG:TTGT

Average Read Length

Read Count

190006

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9146UAUA

InVitroSystem

Jurkat

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library