IGVFFI4071NQGH

{
    "@context": "/terms/",
    "@id": "/sequence-files/IGVFFI4071NQGH/",
    "@type": [
        "SequenceFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI4071NQGH",
    "aliases": [
        "jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep1-BinB-PCRRep1_I2"
    ],
    "assay_titles": [
        "Variant-EFFECTS"
    ],
    "audit": {},
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization"
    },
    "content_md5sum": "773e51763dbd9095b47501e7f20d32db",
    "content_type": "reads",
    "controlled_access": false,
    "creation_timestamp": "2024-11-22T20:25:38.599566+00:00",
    "externally_hosted": false,
    "file_format": "fastq",
    "file_set": {
        "@id": "/measurement-sets/IGVFDS2419JBTK/",
        "accession": "IGVFDS2419JBTK",
        "file_set_type": "experimental data",
        "samples": [
            {
                "@id": "/in-vitro-systems/IGVFSM9146UAUA/",
                "accession": "IGVFSM9146UAUA",
                "classifications": [
                    "cell line"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                        "modality": "prime editing",
                        "status": "released",
                        "summary": "CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/EFO_0002796/",
                        "status": "released",
                        "term_name": "Jurkat"
                    }
                ],
                "status": "preview",
                "summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
                "taxa": "Homo sapiens",
                "treatments": [
                    {
                        "@id": "/treatments/4b5f0d13-ec20-4b91-85f5-a009cd91451f/",
                        "purpose": "stimulation",
                        "status": "in progress",
                        "summary": "Treatment of 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours",
                        "treatment_term_name": "phorbol 13-acetate 12-myristate"
                    },
                    {
                        "@id": "/treatments/cf87f410-54ee-48ef-b654-8443c4fee032/",
                        "purpose": "stimulation",
                        "status": "in progress",
                        "summary": "Treatment of 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours",
                        "treatment_term_name": "T-cell surface glycoprotein CD3 gamma chain"
                    }
                ]
            }
        ],
        "status": "preview",
        "summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus"
    },
    "file_size": 1520414,
    "gene_list_for": [],
    "href": "/sequence-files/IGVFFI4071NQGH/@@download/IGVFFI4071NQGH.fastq.gz",
    "illumina_read_type": "I2",
    "index": "ACCAACTG:TTGT",
    "input_file_for": [
        "/tabular-files/IGVFFI9903UNGI/"
    ],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "loci_list_for": [],
    "maximum_read_length": 4,
    "md5sum": "3b1403a9ae72d5a393dfeabd45ff38a2",
    "mean_read_length": 4,
    "minimum_read_length": 4,
    "read_count": 190006,
    "s3_uri": "s3://igvf-files/2024/11/22/fc21ebcd-3e4a-4277-be55-4a071978116e/IGVFFI4071NQGH.fastq.gz",
    "schema_version": "14",
    "seqspecs": [],
    "sequencing_platform": {
        "@id": "/platform-terms/EFO_0022840/",
        "status": "released",
        "term_name": "Illumina NovaSeq X"
    },
    "sequencing_run": 1,
    "status": "preview",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_file_name": "Jurkat_Stim-IL2RA_prom-BioRep1-FFRep1-PCRRep1-BinB_S2_I2_001.fastq.gz",
    "summary": "I2 reads from sequencing run 1",
    "upload_status": "validated",
    "uuid": "fc21ebcd-3e4a-4277-be55-4a071978116e"
}