IGVFFI4013TWUP

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS3800XFMC(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:240908-S029-PPIF_5SpliceSitePool-THP1-BioRep1-FFrep1-BinC-PCRrep4_R2
md5sum
d9592ee9e33df4fb7e2c0161d2515f73
Content MD5sum
9bf45f08ce5c773cf0be2b2ebab46dcb
File Size
12 MB
Submitted File Name
240908-S029-5splice-THP1-5splicesite_pool-Amp_PPIF_5splicesite-BioRep1-FFrep1-BinC-PCRrep4_S745_R2_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Illumina Read Type
R2
Index
CAGGAGCC:GATATCGA
Average Read Length
151
Read Count
604632

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
variant effects
variant effects
Jesse Engreitz, Stanford
pending

Attribution