{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI4013TWUP/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI4013TWUP",
"aliases": [
"jesse-engreitz:240908-S029-PPIF_5SpliceSitePool-THP1-BioRep1-FFrep1-BinC-PCRrep4_R2"
],
"assay_titles": [
"Variant-EFFECTS"
],
"audit": {},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"content_md5sum": "9bf45f08ce5c773cf0be2b2ebab46dcb",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-12-01T21:29:32.925874+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
"@id": "/measurement-sets/IGVFDS3800XFMC/",
"accession": "IGVFDS3800XFMC",
"file_set_type": "experimental data",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM5956MLVS/",
"accession": "IGVFSM5956MLVS",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
"modality": "prime editing",
"status": "released",
"summary": "CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0001253/",
"status": "released",
"term_name": "THP-1"
}
],
"status": "preview",
"summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"status": "preview",
"summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus"
},
"file_size": 11717664,
"gene_list_for": [],
"href": "/sequence-files/IGVFFI4013TWUP/@@download/IGVFFI4013TWUP.fastq.gz",
"illumina_read_type": "R2",
"index": "CAGGAGCC:GATATCGA",
"input_file_for": [
"/tabular-files/IGVFFI1798XNPV/"
],
"integrated_in": [],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"loci_list_for": [],
"maximum_read_length": 151,
"md5sum": "d9592ee9e33df4fb7e2c0161d2515f73",
"mean_read_length": 151,
"minimum_read_length": 151,
"read_count": 604632,
"s3_uri": "s3://igvf-files/2024/12/01/28d62753-71bd-4b45-9ace-9f3c72eca0da/IGVFFI4013TWUP.fastq.gz",
"schema_version": "14",
"seqspecs": [],
"sequencing_platform": {
"@id": "/platform-terms/EFO_0022840/",
"status": "released",
"term_name": "Illumina NovaSeq X"
},
"sequencing_run": 1,
"status": "preview",
"submitted_by": {
"@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
"title": "Michael Montgomery"
},
"submitted_file_name": "240908-S029-5splice-THP1-5splicesite_pool-Amp_PPIF_5splicesite-BioRep1-FFrep1-BinC-PCRrep4_S745_R2_001.fastq.gz",
"summary": "R2 reads from sequencing run 1",
"upload_status": "validated",
"uuid": "28d62753-71bd-4b45-9ace-9f3c72eca0da"
}