Homo sapiens CD8-positive, alpha-beta memory T primary cell primary cell, modified with CRISPRko Sp-Cas9, transfected with a expression vector library, guide library, sorted on expression of IL7R
released
AuxiliarySet
IGVFDS2606RJRX
gRNA sequencing
Charles Gersbach, Duke
Summary
gRNA sequencing
revoked
MeasurementSet
IGVFDS3910ZTSZ
low FACS signal CRISPR knockout FACS screen sorted on the expression of IL7R integrating an expression vector library of BATF3 and a guide (sgRNA) library targeting transcription start sites in many genes
Charles Gersbach, Duke
Sample Summary
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)