MAGeCK CRISPR screen workflow

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Luca Pinello

Comprehensive characterization of variants underlying heart and blood diseases with CRISPR base editing

Guillaume Lettre, MHI

TeloHAEC cell line, modified with CRISPR modifications, transfected with a guide library, sorted on expression of NFKBIA

TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 80-100% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library

TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 60-80% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library

TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 0-20% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library

TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 20-40% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library

CRISPR interference FlowFISH screen, Proliferation CRISPR interference screen targeting NFKBIA: guide quantifications

Accession

Aliases

Alternate Accessions

Assay Term

Assemblies

Assessed Genes

Associated Phenotypes

Award

Description

Donors

Taxa

File Set Type

Measured Mechanism

Input File Sets

Measurement Sets

Model Name

Prediction Objects

Preferred Assay Title

Protocols

Sample Summary

Samples

Prediction Scope

Selection Criteria

Sequencing Library Types

Genes

Status

Summary

Assay Targeted Genes

Transcriptome Annotations

UUID

FileSet

File Set

Sample

Files

Provenance

Quality

Object Type

Construct Library Data

Classification

Modification

File Type

File Format

Construct Library Design

Audit Category: Internal Action

input_file_sets.@id

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