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IGVFDS8141XEJE

released
Aliases
guillaume-lettre:BP_KRAB_flowfish_analysis_set
File Set Type
principal analysis
Summary
CRISPR interference FlowFISH screen, Proliferation CRISPR interference screen targeting NFKBIA: guide quantifications
Workflows
Samples
TeloHAEC cell line, modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library, sorted on expression of NFKBIA
Protocols

Files

2 items
csv
guide quantifications
guide quantifications
Guillaume Lettre, MHI
132 KB
validated
csv
guide quantifications
guide quantifications
Guillaume Lettre, MHI
326 KB
validated

File Association Graph

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File Set
File
AnalysisSet
MeasurementSet

Samples

32 items
Sample Terms
Associated Construct Library Sets
Summary
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 0-20% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 0-20% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 20-40% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 20-40% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 20-40% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 60-80% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 20-40% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 60-80% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 20-40% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 60-80% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_teloHAEC
female
Homo sapiens

Associated Construct Library Sets

2 items
Summary
Library Design Files
guide (sgRNA) library targeting phenotype-associated variants genome-wide associated with blood pressure
guide (sgRNA) library targeting phenotype-associated variants genome-wide associated with blood pressure

MeasurementSet Input File Sets

32 items
CRISPR interference FlowFISH screen sorted on the expression of NFKBIA integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide associated with blood pressure
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 60-80% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
CRISPR interference FlowFISH screen sorted on the expression of NFKBIA integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide associated with blood pressure
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 0-20% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
CRISPR interference FlowFISH screen sorted on the expression of NFKBIA integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide associated with blood pressure
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 0-20% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
CRISPR interference FlowFISH screen sorted on the expression of NFKBIA integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide associated with blood pressure
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 80-100% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
CRISPR interference FlowFISH screen sorted on the expression of NFKBIA integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide associated with blood pressure
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 0-20% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
CRISPR interference FlowFISH screen sorted on the expression of NFKBIA integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide associated with blood pressure
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 0-20% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
CRISPR interference FlowFISH screen sorted on the expression of NFKBIA integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide associated with blood pressure
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 80-100% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
CRISPR interference FlowFISH screen sorted on the expression of NFKBIA integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide associated with blood pressure
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 20-40% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
CRISPR interference FlowFISH screen sorted on the expression of NFKBIA integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide associated with blood pressure
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 0-20% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
CRISPR interference FlowFISH screen sorted on the expression of NFKBIA integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide associated with blood pressure
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS 20-40% expression of NFKBIA) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library

Attribution

Award
HG012010
Principal Investigator(s)
Contact P.I.
Luca Pinello
Lab
Guillaume Lettre, MHI