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samples.@id
/primary-cells/IGVFSM8084QKPW/
3 items
IGVFDS2160ZTSJ
  • charles-gersbach:cd8-tcells-measurement-set-crispr-ko-tfome-batf3-over-high-bin-rep1
in vitro CRISPR screen using flow cytometry
experimental data
CRISPR FACS screen
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)
released
CRISPR knockout FACS screen sorted on the expression of IL7R integrating an expression vector library of BATF3 and a guide (sgRNA) library targeting transcription start sites in many genes
IGVFDS3370BGRA
  • charles-gersbach:cd8-tcells-auxiliary-set-crispr-ko-tfome-batf3-over-high-bin-rep1
gRNA sequencing
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)
revoked
gRNA sequencing
IGVFDS8364HJHL
  • charles-gersbach:cd8-tcells-analysis-set-crispr-ko-tfome-batf3-over
principal analysis
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4), Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)
Homo sapiens CD8-positive, alpha-beta memory T primary cell primary cell, modified with CRISPRko Sp-Cas9, transfected with a expression vector library, guide library, sorted on expression of IL7R
released
CRISPR knockout FACS screen targeting IL7R: alignments, fold change over control, guide quantifications