principal analysis

Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Homo sapiens CD8-positive, alpha-beta memory T primary cell primary cell, modified with CRISPRko Sp-Cas9, transfected with a guide library

knockout RNA-seq: fold change over control, transcript quantifications, transcriptome alignments

bulk RNA-seq assay

experimental data

RNA-seq

Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

non-targeting CRISPR knockout RNA-seq integrating a non-targeting guide (sgRNA) library

intermediate analysis

Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Homo sapiens CD8-positive, alpha-beta memory T primary cell primary cell, modified with CRISPRko Sp-Cas9, transfected with a guide library

knockout RNA-seq: transcriptome alignments

principal analysis

Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Homo sapiens CD8-positive, alpha-beta memory T primary cell primary cell, modified with CRISPRko Sp-Cas9, transfected with a guide library

knockout RNA-seq: fold change over control, transcript quantifications, transcriptome alignments