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IGVFDS6924DJJC

released
Aliases
charles-gersbach:cd8-tcells-intermediate-analysis-rna-seq-cas-ko-nt
File Set Type
intermediate analysis
Summary
CRISPR knockout RNA-seq integrating a non-targeting guide (sgRNA) library: transcriptome alignments
Workflows
Publications
Samples
Homo sapiens CD8-positive, alpha-beta memory T primary cell primary cell, modified with CRISPRko Sp-Cas9, transfected with a guide library
Protocols
Attribution
Charles Gersbach, Duke

Files

3 items
bam
transcriptome alignments
GRCh38 filtered transcriptome alignments
Charles Gersbach, Duke
2.7 GB
validated
bam
transcriptome alignments
GRCh38 filtered transcriptome alignments
Charles Gersbach, Duke
2.3 GB
validated
bam
transcriptome alignments
GRCh38 filtered transcriptome alignments
Charles Gersbach, Duke
2.5 GB
validated

File Association Graph

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File Set
File
AnalysisSet
MeasurementSet

Samples

3 items
Sample Terms
Associated Construct Library Sets
Summary
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

3 items
Aliases
  • charles-gersbach:cd8-tcells-donor-14
unspecified
Homo sapiens
  • charles-gersbach:cd8-tcells-donor-13
unspecified
Homo sapiens
  • charles-gersbach:cd8-tcells-donor-12
unspecified
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
non-targeting guide (sgRNA) library

MeasurementSet Input File Sets

3 items
non-targeting CRISPR knockout RNA-seq integrating a non-targeting guide (sgRNA) library
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
non-targeting CRISPR knockout RNA-seq integrating a non-targeting guide (sgRNA) library
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
non-targeting CRISPR knockout RNA-seq integrating a non-targeting guide (sgRNA) library
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

File Sets Using This Analysis Set as an Input

2 items
AnalysisSet
CRISPR knockout RNA-seq integrating a guide (sgRNA) library targeting transcription start sites in GATA3 and a non-targeting guide (sgRNA) library: fold change over control, transcript quantifications, transcriptome alignments
  • charles-gersbach:cd8-tcells-analysis-set-rna-seq-cas-ko-gata3
Charles Gersbach, Duke
AnalysisSet
CRISPR knockout RNA-seq integrating a guide (sgRNA) library targeting transcription start sites in ZNF217 and a non-targeting guide (sgRNA) library: fold change over control, transcript quantifications, transcriptome alignments
  • charles-gersbach:cd8-tcells-analysis-set-rna-seq-cas-ko-znf217
Charles Gersbach, Duke