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Measurement Set
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IGVFDS9915OTKB
IGVFDS9915OTKB
released
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File Set Type
experimental data
Summary
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
cas mediated mutagenesis
Sample Summaries
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with a guide library
Construct Library Sets
IGVFDS2374NXLW
Guide (sgRNA) library targeting sequence variants in a genomic locus
Protocols
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
Aliases
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep3-BinA_AmpliconSequencing
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM4653AWMR
InVitroSystem
THP-1
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with a guide library
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO7359LVPI
igvf:donor_of_THP1
male
Homo sapiens
Sequencing Results (Illumina)
48 items
1
2
3
4
5
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI0015ASLT
fastq
reads
R2
1
Illumina NextSeq 550
AACAATGG:ACTG
3.7 MB
Jesse Engreitz, Stanford
validated
IGVFFI0228KHUN
fastq
reads
I1
1
Illumina NextSeq 550
AACGCATT:ACTG
850 KB
Jesse Engreitz, Stanford
validated
IGVFFI0798GSWP
fastq
reads
R1
1
Illumina NextSeq 550
AACGCATT:ACTG
3.0 MB
Jesse Engreitz, Stanford
validated
IGVFFI0895UIJG
fastq
reads
R1
1
Illumina NextSeq 550
ACTGTATC:ACTG
3.2 MB
Jesse Engreitz, Stanford
validated
IGVFFI0943PFGG
fastq
reads
I1
1
Illumina NextSeq 550
AGGTCGCA:TGAC
774 KB
Jesse Engreitz, Stanford
validated
IGVFFI1055IHQX
fastq
reads
I2
1
Illumina NextSeq 550
AGGTCGCA:ACTG
812 KB
Jesse Engreitz, Stanford
validated
IGVFFI1167GINQ
fastq
reads
I1
1
Illumina NextSeq 550
AACAATGG:TGAC
835 KB
Jesse Engreitz, Stanford
validated
IGVFFI1295AUWL
fastq
reads
R2
1
Illumina NextSeq 550
AACGCATT:ACTG
3.6 MB
Jesse Engreitz, Stanford
validated
IGVFFI1360AWJA
fastq
reads
I1
1
Illumina NextSeq 550
AGGTAAGG:ACTG
932 KB
Jesse Engreitz, Stanford
validated
IGVFFI1484WZPG
fastq
reads
I1
1
Illumina NextSeq 550
ACTGTATC:TGAC
839 KB
Jesse Engreitz, Stanford
validated
Control File Sets
1 item
Accession
Summary
Aliases
Lab
IGVFDS4989HPTW
cas mediated mutagenesis (Variant FlowFISH)
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-THP1-PE2_Exp1_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford
Attribution
Award
HG011972
Principal Investigator(s)
Anshul Kundaje
,
Jesse Engreitz
,
Marlene Rabinovitch
,
Lars Steinmetz
,
Michael Bassik
,
Thomas Quertermous
,
Will Greenleaf
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford