OBI_0003090

Ian Whaling

High-Throughput Functional Annotation of Gene Regulatory Elements and Variants Critical to Complex Cellular Phenotypes

CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

knockout RNA-seq integrating a guide (sgRNA) library targeting transcription start sites genome-wide

knockout RNA-seq: transcriptome alignments

Guide (sgRNA) library targeting transcription start sites genome-wide