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INDEXED
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MeasurementSet
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IGVFDS9674AATW
IGVFDS9674AATW
released
Json view
File Set Type
experimental data
Summary
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
Assay Term
in vitro CRISPR screen using single-cell RNA-seq
Sample Summaries
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
Construct Library Sets
IGVFDS0821YMMV
guide (sgRNA) library targeting transcription start sites in 24 genes
Protocols
https://www.protocols.io/view/perturb-seq-characterizing-regulators-of-t-cell-fu-c5s9y6h6
https://www.protocols.io/view/crispri-tiling-screens-c5rby52n
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispri-gex-rep3
Publications
Transcriptional and epigenetic regulators of human CD8+ T cell function identified through orthogonal CRISPR screens
Sequencing Results (Illumina)
8 items
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI0810KWLC
fastq
reads
R2
3
IGVFFI3361HIQA
Illumina NovaSeq 6000
3
14 GB
Charles Gersbach, Duke
validated
IGVFFI0961HLAP
fastq
reads
R2
3
IGVFFI8015EHXD
Illumina NovaSeq 6000
4
14 GB
Charles Gersbach, Duke
validated
IGVFFI2055FUEP
fastq
reads
R2
3
IGVFFI1992VILF
Illumina NovaSeq 6000
1
14 GB
Charles Gersbach, Duke
validated
IGVFFI3515IWOR
fastq
reads
R1
3
IGVFFI9664FBRY
Illumina NovaSeq 6000
2
5.7 GB
Charles Gersbach, Duke
validated
IGVFFI6382YKWB
fastq
reads
R1
3
IGVFFI8015EHXD
Illumina NovaSeq 6000
4
5.7 GB
Charles Gersbach, Duke
validated
IGVFFI7880KEAK
fastq
reads
R1
3
IGVFFI1992VILF
Illumina NovaSeq 6000
1
5.7 GB
Charles Gersbach, Duke
validated
IGVFFI8510AXNZ
fastq
reads
R2
3
IGVFFI9664FBRY
Illumina NovaSeq 6000
2
14 GB
Charles Gersbach, Duke
validated
IGVFFI8540PIGY
fastq
reads
R1
3
IGVFFI3361HIQA
Illumina NovaSeq 6000
3
5.7 GB
Charles Gersbach, Duke
validated
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM5189UBOI
PrimaryCell
CD8-positive, alpha-beta memory T cell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO4425KRAT
charles-gersbach:cd8-tcells-donor-8
unspecified
Homo sapiens
File Sets Using This Measurement Set as an Input
1 item
Accession
Type
Summary
Aliases
Lab
IGVFDS3319RFFN
AnalysisSet
interference Perturb-seq: filtered feature barcode matrix
charles-gersbach:cd8-tcells-intermediate-analysis-set-perturb-seq-crispri
Charles Gersbach, Duke
Auxiliary Datasets
1 item
Accession
Type
Summary
Files
Aliases
Lab
IGVFDS0591VNKJ
AuxiliarySet
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
IGVFFI3970QIWD
,
IGVFFI2219JWDR
,
IGVFFI8824YLDU
,
IGVFFI9916XQDH
,
IGVFFI2934KNNM
,
IGVFFI2856WURS
,
IGVFFI7659OLXW
,
IGVFFI6670OLVF
charles-gersbach:cd8-tcells-perturb-seq-crispri-grna-rep3
Charles Gersbach, Duke
Attribution
Award
HG012053
Principal Investigator(s)
Charles Gersbach
,
Gregory Crawford
,
Tim Reddy
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke